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1d33

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{{STRUCTURE_1d33| PDB=1d33 | SCENE= }}
{{STRUCTURE_1d33| PDB=1d33 | SCENE= }}
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'''FORMALDEHYDE CROSS-LINKS DAUNORUBICIN AND DNA EFFICIENTLY: HPLC AND X-RAY DIFFRACTION STUDIES'''
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===FORMALDEHYDE CROSS-LINKS DAUNORUBICIN AND DNA EFFICIENTLY: HPLC AND X-RAY DIFFRACTION STUDIES===
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==Overview==
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Formaldehyde (HCHO) cross-links the anticancer drug daunorubicin (DAU) to DNA efficiently. When DAU is mixed with DNA hexamers, d(CGCGCG) and d(CGTDCG), in the presence of HCHO, stable covalent adducts of DNA are formed, as shown by the HPLC analyses. The major adducts are identical with the materials in the respective crystals which can be readily obtained from the 1:1 mixture of DAU-d(CGCGCG) and DAU-d(CGTDCG) plus HCHO, but not from the solution without HCHO. The high-resolution (1.5 A) X-ray crystal structure of those adducts shows unambiguously that they contain a covalent methylene bridge between the N3' of daunosamine and the N2 of the guanine or 2-aminoadenine. The perfect juxtaposition of the two amino groups in the minor groove of the complex provides a template for an efficient addition of HCHO. The methylene bridge does not perturb the conformation of the drug-DNA complex, when compared to the structure of DAU-d(CGTACG). The results suggest new approaches for synthesizing a new type of potential anticancer drug by attaching a reactive (e.g., alkylating) functional group at the N3' amino position of daunorubicin/doxorubicin. The stable drug-DNA adduct may be useful as probes for other biological studies.
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{{ABSTRACT_PUBMED_2018756}}
==About this Structure==
==About this Structure==
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[[Category: Modified]]
[[Category: Modified]]
[[Category: Right handed dna]]
[[Category: Right handed dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:23:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:15:56 2008''

Revision as of 19:15, 30 June 2008

Image:1d33.png

Template:STRUCTURE 1d33

FORMALDEHYDE CROSS-LINKS DAUNORUBICIN AND DNA EFFICIENTLY: HPLC AND X-RAY DIFFRACTION STUDIES

Template:ABSTRACT PUBMED 2018756

About this Structure

Full crystallographic information is available from OCA.

Reference

Formaldehyde cross-links daunorubicin and DNA efficiently: HPLC and X-ray diffraction studies., Wang AH, Gao YG, Liaw YC, Li YK, Biochemistry. 1991 Apr 23;30(16):3812-5. PMID:2018756

Page seeded by OCA on Mon Jun 30 22:15:56 2008

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