1z9e

From Proteopedia

Revision as of 18:24, 12 November 2007

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75

Overview

Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding, partner of HIV-1 integrase (IN) in human cells. We have determined the NMR, structure of the integrase-binding domain (IBD) in LEDGF and identified, amino acid residues essential for the interaction. The IBD is a compact, right-handed bundle composed of five alpha-helices. Based on folding, topology, the IBD is structurally related to a diverse family of, alpha-helical proteins that includes eukaryotic translation initiation, factor eIF4G and karyopherin-beta. LEDGF residues essential for the, interaction with IN were localized to interhelical loop regions of the, bundle structure. Interaction-defective IN mutants were previously shown, to cripple replication although they retained catalytic function. The, initial structure determination of a host cell factor that tightly binds, to a retroviral enzyme lays the groundwork for understanding enzyme-host, interactions important for viral replication.

About this Structure

1Z9E is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75., Cherepanov P, Sun ZY, Rahman S, Maertens G, Wagner G, Engelman A, Nat Struct Mol Biol. 2005 Jun;12(6):526-32. Epub 2005 May 15. PMID:15895093

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