1agt

<StructureSection load='1agt' size='340' side='right'caption='[[1agt]], [[NMR_Ensembles_of_Models | 17 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[1agt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiqh Leiqh]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1AGT FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGTX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 LEIQH])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1agt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1agt OCA], [http://pdbe.org/1agt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1agt RCSB], [http://www.ebi.ac.uk/pdbsum/1agt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1agt ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/KAX32_LEIQH KAX32_LEIQH]] Potent inhibitor of shaker potassium channels as well as the mammalian homologs of shaker.

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== Publication Abstract from PubMed ==

The structure of the potassium channel blocker agitoxin 2 was solved by solution NMR methods. The structure consists of a triple-stranded antiparallel beta-sheet and a single helix covering one face of the beta-sheet. The cysteine side chains connecting the beta-sheet and the helix form the core of the molecule. One edge of the beta-sheet and the adjacent face of the helix form the interface with the Shaker K+ channel. The fold of agitoxin is homologous to the previously determined folds of scorpion venom toxins. However, agitoxin 2 differs significantly from the other channel blockers in the specificity of its interactions. This study was thus focused on a precise characterization of the surface residues at the face of the protein interacting with the Shaker K+ channel. The rigid toxin molecule can be used to estimate dimensions of the potassium channel. Surface-exposed residues, Arg24, Lys27, and Arg31 of the beta-sheet, have been identified from mutagenesis studies as functionally important for blocking the Shaker K+ channel. The sequential and spatial locations of Arg24 and Arg31 are not conserved among the homologous toxins. Knowledge on the details of the channel-binding sites of agitoxin 2 formed a basis for site-directed mutagenesis studies of the toxin and the K+ channel sequences. Observed interactions between mutated toxin and channel are being used to elucidate the channel structure and mechanisms of channel-toxin interactions.

Solution structure of the potassium channel inhibitor agitoxin 2: caliper for probing channel geometry.,Krezel AM, Kasibhatla C, Hidalgo P, MacKinnon R, Wagner G Protein Sci. 1995 Aug;4(8):1478-89. PMID:8520473<ref>PMID:8520473</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1agt" style="background-color:#fffaf0;"></div>

[[Category: Leiqh]]

[[Category: Hidalgo, P]]

[[Category: Kasibhatla, C]]

[[Category: Krezel, A M]]

[[Category: Mackinnon, R]]

[[Category: Wagner, G]]

[[Category: Potassium channel blocker]]