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| | <StructureSection load='1ebu' size='340' side='right'caption='[[1ebu]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='1ebu' size='340' side='right'caption='[[1ebu]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ebu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1EBU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ebu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EBU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HSE:L-HOMOSERINE'>HSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NDA:3-AMINOMETHYL-PYRIDINIUM-ADENINE-DINUCLEOTIDE'>NDA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ebf|1ebf]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HSE:L-HOMOSERINE'>HSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NDA:3-AMINOMETHYL-PYRIDINIUM-ADENINE-DINUCLEOTIDE'>NDA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HOM6P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ebu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ebu OCA], [https://pdbe.org/1ebu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ebu RCSB], [https://www.ebi.ac.uk/pdbsum/1ebu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ebu ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] </span></td></tr> | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ebu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ebu OCA], [http://pdbe.org/1ebu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ebu RCSB], [http://www.ebi.ac.uk/pdbsum/1ebu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ebu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DHOM_YEAST DHOM_YEAST] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| - | [[Category: Homoserine dehydrogenase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Berghuis, A M]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: DeLaBarre, B]] | + | [[Category: Berghuis AM]] |
| - | [[Category: Thompson, P R]] | + | [[Category: DeLaBarre B]] |
| - | [[Category: Wright, G D]] | + | [[Category: Thompson PR]] |
| - | [[Category: Analogue]] | + | [[Category: Wright GD]] |
| - | [[Category: Dehydrogenase]]
| + | |
| - | [[Category: Dinucleotide]]
| + | |
| - | [[Category: Homoserine]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Ternary]]
| + | |
| Structural highlights
Function
DHOM_YEAST
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases.,DeLaBarre B, Thompson PR, Wright GD, Berghuis AM Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:10700284[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ DeLaBarre B, Thompson PR, Wright GD, Berghuis AM. Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases. Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:10700284 doi:10.1038/73359
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