This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1d3r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1d3r.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1d3r.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1d3r| PDB=1d3r | SCENE= }}
{{STRUCTURE_1d3r| PDB=1d3r | SCENE= }}
-
'''CRYSTAL STRUCTURE OF TRIPLEX DNA'''
+
===CRYSTAL STRUCTURE OF TRIPLEX DNA===
-
==Overview==
+
<!--
-
Extended purine sequences on a DNA strand can lead to the formation of triplex DNA in which the third strand runs parallel to the purine strand. Triplex DNA structures have been proposed to play a role in gene expression and recombination and also have potential application as antisense inhibitors of gene expression. Triplex structures have been studied in solution by NMR, but have hitherto resisted attempts at crystallization. Here, we report a novel design of DNA sequences, which allows the first crystallographic study of DNA segment containing triplexes and its junction with a duplex. In the 1.8 A resolution structure, the sugar-phosphate backbone of the third strand is parallel to the purine-rich strand. The bases of the third strand associate with the Watson and Crick duplex via Hoogsteen-type interactions, resulting in three consecutive C(+).GC, BU.ABU (BU = 5-bromouracil), and C(+).GC triplets. The overall conformation of the DNA triplex has some similarity to the B-form, but is distinct from both A- and B-forms. There are large changes in the phosphate backbone torsion angles (particularly gamma) of the purine strand, probably due to the electrostatic interactions between the phosphate groups and the protonated cytosine. These changes narrow the minor groove width of the purine-Hoogsteen strands and may represent sequence-specific structural variations of the DNA triplex.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10606513}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10606513 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10606513}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: Rhee, S.]]
[[Category: Rhee, S.]]
[[Category: Triplex dna and its junction with a duplex dna]]
[[Category: Triplex dna and its junction with a duplex dna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:25:10 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:20:09 2008''

Revision as of 19:20, 30 June 2008

Image:1d3r.png

Template:STRUCTURE 1d3r

CRYSTAL STRUCTURE OF TRIPLEX DNA

Template:ABSTRACT PUBMED 10606513

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure of a triple helical DNA with a triplex-duplex junction., Rhee S, Han Z, Liu K, Miles HT, Davies DR, Biochemistry. 1999 Dec 21;38(51):16810-5. PMID:10606513

Page seeded by OCA on Mon Jun 30 22:20:09 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1d3r"
Personal tools