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4a78

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Revision as of 05:27, 25 August 2022

cytochrome c peroxidase M119W in complex with guiacol

Structural highlights

4a78 is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1a2f, 1a2g, 1aa4, 1ac4, 1ac8, 1aeb, 1aed, 1aee, 1aef, 1aeg, 1aeh, 1aej, 1aek, 1aem, 1aen, 1aeo, 1aeq, 1aes, 1aet, 1aeu, 1aev, 1bej, 1bek, 1bem, 1bep, 1beq, 1bes, 1bj9, 1bva, 1cca, 1ccb, 1ccc, 1cce, 1ccg, 1cci, 1ccj, 1cck, 1ccl, 1ccp, 1cmp, 1cmq, 1cmt, 1cmu, 1cpd, 1cpe, 1cpf, 1cpg, 1cyf, 1dcc, 1dj1, 1dj5, 1ds4, 1dse, 1dsg, 1dso, 1dsp, 1ebe, 1jci, 1jdr, 1kok, 1krj, 1kxm, 1kxn, 1mk8, 1mkq, 1mkr, 1ml2, 1ryc, 1s6v, 1sdq, 1sog, 1stq, 1u74, 1u75, 1z53, 1zby, 1zbz, 2b0z, 2b10, 2b11, 2b12, 2bcn, 2ccp, 2cep, 2cyp, 2gb8, 2pcb, 2pcc, 2v23, 2v2e, 2x07, 2x08, 2xil, 2xj5, 2xj8, 2y5a, 2ycg, 3ccp, 3ccx, 4a6z, 4a71, 4a7m, 4ccp, 4ccx, 5ccp, 6ccp, 7ccp
Activity:	Cytochrome-c peroxidase, with EC number 1.11.1.5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CCPR_YEAST] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Publication Abstract from PubMed

Guaiacol is a universal substrate for all peroxidases, and its use in a simple colorimetric assay has wide applications. However, its exact binding location has never been defined. Here we report the crystal structures of guaiacol bound to cytochrome c peroxidase (CcP). A related structure with phenol bound is also presented. The CcP-guaiacol and CcP-phenol crystal structures show that both guaiacol and phenol bind at sites distinct from the cytochrome c binding site and from the delta-heme edge, which is known to be the binding site for other substrates. Although neither guaiacol nor phenol is seen bound at the delta-heme edge in the crystal structures, inhibition data and mutagenesis strongly suggest that the catalytic binding site for aromatic compounds is the delta-heme edge in CcP. The functional implications of these observations are discussed in terms of our existing understanding of substrate binding in peroxidases [Gumiero A et al. (2010) Arch Biochem Biophys 500, 13-20].

Crystal structure of guaiacol and phenol bound to a heme peroxidase.,Murphy EJ, Metcalfe CL, Nnamchi C, Moody PC, Raven EL FEBS J. 2012 May;279(9):1632-9. doi: 10.1111/j.1742-4658.2011.08425.x. Epub 2011 , Dec 5. PMID:22093282^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murphy EJ, Metcalfe CL, Nnamchi C, Moody PC, Raven EL. Crystal structure of guaiacol and phenol bound to a heme peroxidase. FEBS J. 2012 May;279(9):1632-9. doi: 10.1111/j.1742-4658.2011.08425.x. Epub 2011 , Dec 5. PMID:22093282 doi:http://dx.doi.org/10.1111/j.1742-4658.2011.08425.x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a78"

@@ Line 3: / Line 3: @@
 <StructureSection load='4a78' size='340' side='right'caption='[[4a78]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4a78]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A78 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4A78 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4a78]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A78 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=JZ3:GUAIACOL'>JZ3</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1a2f|1a2f]], [[1a2g|1a2g]], [[1aa4|1aa4]], [[1ac4|1ac4]], [[1ac8|1ac8]], [[1aeb|1aeb]], [[1aed|1aed]], [[1aee|1aee]], [[1aef|1aef]], [[1aeg|1aeg]], [[1aeh|1aeh]], [[1aej|1aej]], [[1aek|1aek]], [[1aem|1aem]], [[1aen|1aen]], [[1aeo|1aeo]], [[1aeq|1aeq]], [[1aes|1aes]], [[1aet|1aet]], [[1aeu|1aeu]], [[1aev|1aev]], [[1bej|1bej]], [[1bek|1bek]], [[1bem|1bem]], [[1bep|1bep]], [[1beq|1beq]], [[1bes|1bes]], [[1bj9|1bj9]], [[1bva|1bva]], [[1cca|1cca]], [[1ccb|1ccb]], [[1ccc|1ccc]], [[1cce|1cce]], [[1ccg|1ccg]], [[1cci|1cci]], [[1ccj|1ccj]], [[1cck|1cck]], [[1ccl|1ccl]], [[1ccp|1ccp]], [[1cmp|1cmp]], [[1cmq|1cmq]], [[1cmt|1cmt]], [[1cmu|1cmu]], [[1cpd|1cpd]], [[1cpe|1cpe]], [[1cpf|1cpf]], [[1cpg|1cpg]], [[1cyf|1cyf]], [[1dcc|1dcc]], [[1dj1|1dj1]], [[1dj5|1dj5]], [[1ds4|1ds4]], [[1dse|1dse]], [[1dsg|1dsg]], [[1dso|1dso]], [[1dsp|1dsp]], [[1ebe|1ebe]], [[1jci|1jci]], [[1jdr|1jdr]], [[1kok|1kok]], [[1krj|1krj]], [[1kxm|1kxm]], [[1kxn|1kxn]], [[1mk8|1mk8]], [[1mkq|1mkq]], [[1mkr|1mkr]], [[1ml2|1ml2]], [[1ryc|1ryc]], [[1s6v|1s6v]], [[1sdq|1sdq]], [[1sog|1sog]], [[1stq|1stq]], [[1u74|1u74]], [[1u75|1u75]], [[1z53|1z53]], [[1zby|1zby]], [[1zbz|1zbz]], [[2b0z|2b0z]], [[2b10|2b10]], [[2b11|2b11]], [[2b12|2b12]], [[2bcn|2bcn]], [[2ccp|2ccp]], [[2cep|2cep]], [[2cyp|2cyp]], [[2gb8|2gb8]], [[2pcb|2pcb]], [[2pcc|2pcc]], [[2v23|2v23]], [[2v2e|2v2e]], [[2x07|2x07]], [[2x08|2x08]], [[2xil|2xil]], [[2xj5|2xj5]], [[2xj8|2xj8]], [[2y5a|2y5a]], [[2ycg|2ycg]], [[3ccp|3ccp]], [[3ccx|3ccx]], [[4a6z|4a6z]], [[4a71|4a71]], [[4a7m|4a7m]], [[4ccp|4ccp]], [[4ccx|4ccx]], [[5ccp|5ccp]], [[6ccp|6ccp]], [[7ccp|7ccp]]</div></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] </span></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4a78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a78 OCA], [http://pdbe.org/4a78 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a78 RCSB], [http://www.ebi.ac.uk/pdbsum/4a78 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a78 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a78 OCA], [https://pdbe.org/4a78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a78 RCSB], [https://www.ebi.ac.uk/pdbsum/4a78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a78 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CCPR_YEAST CCPR_YEAST]] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
+[[https://www.uniprot.org/uniprot/CCPR_YEAST CCPR_YEAST]] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

4a78

From Proteopedia

Revision as of 05:27, 25 August 2022

cytochrome c peroxidase M119W in complex with guiacol

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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