1d5n

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{{Seed}}
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{{STRUCTURE_1d5n| PDB=1d5n | SCENE= }}
{{STRUCTURE_1d5n| PDB=1d5n | SCENE= }}
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'''CRYSTAL STRUCTURE OF E. COLI MNSOD AT 100K'''
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===CRYSTAL STRUCTURE OF E. COLI MNSOD AT 100K===
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==Overview==
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Superoxide dismutase protects organisms from potentially damaging oxygen radicals by catalyzing the disproportionation of superoxide to oxygen and hydrogen peroxide. We report the use of cryogenic temperatures to kinetically capture the sixth ligand bound to the active site of manganese superoxide dismutase (MnSOD). Synchrotron X-ray diffraction data was collected from Escherichia coli MnSOD crystals grown at pH 8.5 and cryocooled to 100 K. Structural refinement to 1.55 A resolution and close inspection of the active site revealed electron density for a sixth ligand that was interpreted to be a hydroxide ligand. The six-coordinate, distorted-octahedral geometry assumed during inhibition by hydroxide is compared to the room temperature, five-coordinate, trigonal bipyramidal active site determined with crystals grown from practically identical conditions. The gateway residues Tyr34, His30 and a tightly bound water molecule are implicated in closing-off the active site and blocking the escape route of the sixth ligand.
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(as it appears on PubMed at http://www.pubmed.gov), where 10686094 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10686094}}
==About this Structure==
==About this Structure==
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[[Category: Manganese superoxide dismutase]]
[[Category: Manganese superoxide dismutase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:28:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:29:04 2008''

Revision as of 19:29, 30 June 2008

Image:1d5n.png

Template:STRUCTURE 1d5n

CRYSTAL STRUCTURE OF E. COLI MNSOD AT 100K

Template:ABSTRACT PUBMED 10686094

About this Structure

1D5N is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Cryo-trapping the six-coordinate, distorted-octahedral active site of manganese superoxide dismutase., Borgstahl GE, Pokross M, Chehab R, Sekher A, Snell EH, J Mol Biol. 2000 Mar 3;296(4):951-9. PMID:10686094

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