1d6g

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[[Image:1d6g.gif|left|200px]]
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{{STRUCTURE_1d6g| PDB=1d6g | SCENE= }}
{{STRUCTURE_1d6g| PDB=1d6g | SCENE= }}
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'''MOLECULAR COMPLEX OF CHOLECYSTOKININ-8 AND N-TERMINUS OF THE CHOLECYSTOKININ A RECEPTOR BY NMR SPECTROSCOPY'''
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===MOLECULAR COMPLEX OF CHOLECYSTOKININ-8 AND N-TERMINUS OF THE CHOLECYSTOKININ A RECEPTOR BY NMR SPECTROSCOPY===
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==Overview==
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The bimolecular complex of the C-terminal octapeptide of cholecystokinin, CCK-8, with the N-terminus of the CCK(A)-receptor, CCK(A)-R(1-47), has been structurally characterized by high-resolution NMR and computational refinement. The conformation of CCK(A)-R(1-47), within the lipid environment used for the spectroscopic studies, consists of a well-defined alpha-helix (residues 3-9) followed by a beta-sheet stabilized by a disulfide linkage between C18 and C29, leading to the first transmembrane alpha-helix (TM1). Titration of CCK(A)-R(1-47) with CCK-8 specifically affects the NMR signals of W39 of the receptor, in a saturable fashion. This association is specific for CCK-8; no association was observed upon titration of CCK(A)-R(1-47) with other peptide hormones. The ligand/receptor complex was characterized by intermolecular NOEs between Tyr(27) and Met(28) of CCK-8 and W39 of CCK(A)-R(1-47). These findings suggest that CCK-8 binds to CCK(A) with the C-terminus within the seven-helical bundle and the N-terminus of the ligand, projecting out between TM1 and TM7, forming specific interactions with the N-terminus of the CCK(A) receptor. This mode of ligand binding, consistent with published mutagenesis studies, requires variation of the interpretation of recent findings from photoaffinity cross-linking studies.
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(as it appears on PubMed at http://www.pubmed.gov), where 10555959 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10555959}}
==About this Structure==
==About this Structure==
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1D6G is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6G OCA].
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1D6G is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6G OCA].
==Reference==
==Reference==
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[[Category: Beta-sheet]]
[[Category: Beta-sheet]]
[[Category: Complex gpcr-ligand]]
[[Category: Complex gpcr-ligand]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:30:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:32:07 2008''

Revision as of 19:32, 30 June 2008

Image:1d6g.png

Template:STRUCTURE 1d6g

MOLECULAR COMPLEX OF CHOLECYSTOKININ-8 AND N-TERMINUS OF THE CHOLECYSTOKININ A RECEPTOR BY NMR SPECTROSCOPY

Template:ABSTRACT PUBMED 10555959

About this Structure

1D6G is a Single protein structure. Full experimental information is available from OCA.

Reference

Molecular complex of cholecystokinin-8 and N-terminus of the cholecystokinin A receptor by NMR spectroscopy., Pellegrini M, Mierke DF, Biochemistry. 1999 Nov 9;38(45):14775-83. PMID:10555959

Page seeded by OCA on Mon Jun 30 22:32:07 2008

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