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6lkl
From Proteopedia
(Difference between revisions)
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==Two-component system protein mediate signal transduction== | ==Two-component system protein mediate signal transduction== | ||
| - | <StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]]' scene=''> | + | <StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]], [[Resolution|resolution]] 2.21Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lkl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [http://pdbe.org/6lkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [http://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">E4U00_07700, EP54_06205, EQ90_12025, HMPREF3211_02751, NCTC10654_00249, NCTC10702_00414, RK64_01575 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [http://pdbe.org/6lkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [http://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Two-component systems (TCS), which typically consist of a membrane-embedded histidine kinase and a cytoplasmic response regulator, are the dominant signaling proteins for transduction of environmental stimuli into cellular response pathways in prokaryotic cells. HptRSA is a recently identified TCS consisting of the G6P-associated sensor protein (HptA), transmembrane histidine kinase (HptS), and cytoplasmic effector (HptR). HptRSA mediates glucose-6-phosphate (G6P) uptake to support Staphylococcus aureus growth and multiplication within various host cells. How the mechanism by which HptRSA perceives G6P and triggers a downstream response has remained elusive. Here, we solved the HptA structures in apo and G6P-bound states. G6P binding in the cleft between two HptA domains caused a conformational closing movement. The solved structures of HptA in complex with the periplasmic domain of HptS showed that HptA interacts with HptS through both constitutive and switchable interfaces. The G6P-free form of HptA binds to the membrane-distal side of the HptS periplasmic domain (HptSp), resulting in a parallel conformation of the HptSp protomer pair. However, once HptA associates with G6P, its intramolecular domain closure switches the HptA-HptSp contact region into the membrane-proximal domain, which causes rotation and closure of the C termini of each HptSp protomer. Through biochemical and growth assays of HptA and HptS mutant variants, we proposed a distinct mechanism of interface switch-mediated signaling transduction. Our results provide mechanistic insights into bacterial nutrient sensing and expand our understanding of the activation modes by which TCS communicates external signals. | ||
| + | |||
| + | Interface switch mediates signal transmission in a two-component system.,Wang M, Guo Q, Zhu K, Fang B, Yang Y, Teng M, Li X, Tao Y Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30433-30440. doi:, 10.1073/pnas.1912080117. Epub 2020 Nov 16. PMID:33199635<ref>PMID:33199635</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lkl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Tao Y]] | + | [[Category: Tao, Y]] |
| - | [[Category: Wang M]] | + | [[Category: Wang, M]] |
| + | [[Category: G6p sensor]] | ||
| + | [[Category: Signal transduction]] | ||
| + | [[Category: Signaling protein]] | ||
| + | [[Category: Two-component system]] | ||
Revision as of 12:41, 16 December 2020
Two-component system protein mediate signal transduction
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