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| - | [[Image:1d6s.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1d6s| PDB=1d6s | SCENE= }} | | {{STRUCTURE_1d6s| PDB=1d6s | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE'''
| + | ===CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE=== |
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| - | ==Overview==
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| - | Covalent binding of L-methionine as an external aldimine to the pyridoxal 5'-phosphate-cofactor in the K41A mutant of O-acetylserine sulfhydrylase from Salmonella typhimurium induces a large conformational change in the protein. Methionine mimics the action of the substrate O-acetyl-L-serine during catalysis. The alpha-carboxylate moiety of L-methionine in external aldimine linkage with the active site pyridoxal 5'-phosphate forms a hydrogen bonding network to the "asparagine-loop" P67-T68-N69-G70 which adopts a different conformation than in the native protein. The side-chain nitrogen of Asn69 moves more than 7 A to make a hydrogen bond to the alpha-carboxylate group of the inhibitor. As the external aldimine is formed, the PLP tilts by 13 degrees along its longitudinal axis such that C4' moves toward the entrance to the active site and the side-chain of the methionine is directed toward the active site entrance. The local rearrangement acts as a trigger to induce a large global conformational change in the protein. A subdomain comprised of beta-strand 4, alpha-helix 3, beta-strand 5 and alpha-helix 4 moves towards the active site by a rotation of 7 degrees. This subdomain movement results in a reduction of the severe twist of its central beta-sheet and reduces the active site entrance to a small hole, giving access only to small molecules like sulfide, the second substrate, or acetate, the first product.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10452898}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10452898 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10452898}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: K41a]] | | [[Category: K41a]] |
| | [[Category: Plp]] | | [[Category: Plp]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:30:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:33:07 2008'' |
Revision as of 19:33, 30 June 2008
Template:STRUCTURE 1d6s
CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE
Template:ABSTRACT PUBMED 10452898
About this Structure
1D6S is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Ligand binding induces a large conformational change in O-acetylserine sulfhydrylase from Salmonella typhimurium., Burkhard P, Tai CH, Ristroph CM, Cook PF, Jansonius JN, J Mol Biol. 1999 Aug 27;291(4):941-53. PMID:10452898
Page seeded by OCA on Mon Jun 30 22:33:07 2008
