6zzz
From Proteopedia
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==Crystal structure of yeast Sec62 cytoplasmic domain== | ==Crystal structure of yeast Sec62 cytoplasmic domain== | ||
| - | <StructureSection load='6zzz' size='340' side='right'caption='[[6zzz]]' scene=''> | + | <StructureSection load='6zzz' size='340' side='right'caption='[[6zzz]], [[Resolution|resolution]] 2.54Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZZZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZZZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zzz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZZZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZZZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zzz OCA], [http://pdbe.org/6zzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zzz RCSB], [http://www.ebi.ac.uk/pdbsum/6zzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zzz ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEC62, YPL094C, LPG14C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zzz OCA], [http://pdbe.org/6zzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zzz RCSB], [http://www.ebi.ac.uk/pdbsum/6zzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zzz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SEC62_YEAST SEC62_YEAST]] Acts as component of the Sec62/63 complex which is involved in SRP-independent post-translational translocation across the endoplasmic reticulum (ER) and functions together with the Sec61 complex and KAR2 in a channel-forming translocon complex. In an initial step, the signal sequence seems to bind simultaneously to SEC61 and SEC62. SEC62 and SEC63 are required for interactions between SEC61 and translocating polypeptides. SEC62 may affect SEC1-polypeptide interactions by increasing the affinity of targeting pathways for SEC61 and/or by modifying SEC61 to allow more efficient polypeptide interaction. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC62 is essential for cell growth. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In eukaryotes, most secretory and membrane proteins are targeted by an N-terminal signal sequence to the endoplasmic reticulum, where the trimeric Sec61 complex serves as protein-conducting channel (PCC). In the post-translational mode, fully synthesized proteins are recognized by a specialized channel additionally containing the Sec62, Sec63, Sec71, and Sec72 subunits. Recent structures of this Sec complex in the idle state revealed the overall architecture in a pre-opened state. Here, we present a cryo-EM structure of the yeast Sec complex bound to a substrate, and a crystal structure of the Sec62 cytosolic domain. The signal sequence is inserted into the lateral gate of Sec61alpha similar to previous structures, yet, with the gate adopting an even more open conformation. The signal sequence is flanked by two Sec62 transmembrane helices, the cytoplasmic N-terminal domain of Sec62 is more rigidly positioned, and the plug domain is relocated. We crystallized the Sec62 domain and mapped its interaction with the C-terminus of Sec63. Together, we obtained a near-complete and integrated model of the active Sec complex. | ||
| + | |||
| + | Architecture of the active post-translational Sec translocon.,Weng TH, Steinchen W, Beatrix B, Berninghausen O, Becker T, Bange G, Cheng J, Beckmann R EMBO J. 2020 Dec 11:e105643. doi: 10.15252/embj.2020105643. PMID:33305433<ref>PMID:33305433</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zzz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Baker's yeast]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Beckmann R]] | + | [[Category: Beckmann, R]] |
| - | [[Category: Cheng J]] | + | [[Category: Cheng, J]] |
| + | [[Category: Post translocon]] | ||
| + | [[Category: Protein transport]] | ||
| + | [[Category: Sec62]] | ||
| + | [[Category: Sec62 domain]] | ||
Revision as of 07:01, 30 December 2020
Crystal structure of yeast Sec62 cytoplasmic domain
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