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1d7a

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{{STRUCTURE_1d7a| PDB=1d7a | SCENE= }}
{{STRUCTURE_1d7a| PDB=1d7a | SCENE= }}
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'''CRYSTAL STRUCTURE OF E. COLI PURE-MONONUCLEOTIDE COMPLEX.'''
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===CRYSTAL STRUCTURE OF E. COLI PURE-MONONUCLEOTIDE COMPLEX.===
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==Overview==
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BACKGROUND: Conversion of 5-aminoimidazole ribonucleotide (AIR) to 4-carboxyaminoimidazole ribonucleotide (CAIR) in Escherichia coli requires two proteins - PurK and PurE. PurE has recently been shown to be a mutase that catalyzes the unusual rearrangement of N(5)-carboxyaminoimidazole ribonucleotide (N(5)-CAIR), the PurK reaction product, to CAIR. PurEs from higher eukaryotes are homologous to E. coli PurE, but use AIR and CO(2) as substrates to produce CAIR directly. RESULTS: The 1.50 A crystal structure of PurE reveals an octameric structure with 422 symmetry. A central three-layer (alphabetaalpha) sandwich domain and a kinked C-terminal helix form the folded structure of the monomeric unit. The structure reveals a cleft at the interface of two subunits and near the C-terminal helix of a third subunit. Co-crystallization experiments with CAIR confirm this to be the mononucleotide-binding site. The nucleotide is bound predominantly to one subunit, with conserved residues from a second subunit making up one wall of the cleft. CONCLUSIONS: The crystal structure of PurE reveals a unique quaternary structure that confirms the octameric nature of the enzyme. An analysis of the native crystal structure, in conjunction with sequence alignments and studies of co-crystals of PurE with CAIR, reveals the location of the active site. The environment of the active site and the analysis of conserved residues between the two classes of PurEs suggests a model for the differences in their substrate specificities and the relationship between their mechanisms.
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(as it appears on PubMed at http://www.pubmed.gov), where 10574791 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10574791}}
==About this Structure==
==About this Structure==
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[[Category: Mathews, I I.]]
[[Category: Mathews, I I.]]
[[Category: Stubbe, J.]]
[[Category: Stubbe, J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:36:14 2008''

Revision as of 19:36, 30 June 2008

Image:1d7a.png

Template:STRUCTURE 1d7a

CRYSTAL STRUCTURE OF E. COLI PURE-MONONUCLEOTIDE COMPLEX.

Template:ABSTRACT PUBMED 10574791

About this Structure

1D7A is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway., Mathews II, Kappock TJ, Stubbe J, Ealick SE, Structure. 1999 Nov 15;7(11):1395-406. PMID:10574791

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