7bov
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bov OCA], [http://pdbe.org/7bov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bov RCSB], [http://www.ebi.ac.uk/pdbsum/7bov PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bov ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bov OCA], [http://pdbe.org/7bov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bov RCSB], [http://www.ebi.ac.uk/pdbsum/7bov PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bov ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glycosylation possess prominent biological and pharmacological significance in natural product and drug candidate synthesis. The glycosyltransferase YjiC, discovered from Bacillus subtilis (Bs-YjiC), shows potential applications in drug development due to its wide substrate spectrums. In order to elucidate its catalytic mechanism, we solved the crystal structure of Bs-YjiC, demonstrating that Bs-YjiC adopts a typical GT-B fold consisting of a flexible N-domain and a relatively rigid C-domain. Structural analysis coupled with site-directed mutagenesis studies revealed that site Ser277 was critical for Nucleoside Diphosphate (NDP) recognition, while Glu317, Gln318, Ser128 and Ser129 were crucial for glycosyl moiety recognition. Our results illustrate the structural basis for acceptor promiscuity in Bs-YjiC and provide a starting point for further protein engineering of Bs-YjiC in industrial and pharmaceutical applications. | ||
| + | |||
| + | Structural and biochemical studies of the glycosyltransferase Bs-YjiC from Bacillus subtilis.,Liu B, Zhao C, Xiang Q, Zhao N, Luo Y, Bao R Int J Biol Macromol. 2021 Jan 1;166:806-817. doi: 10.1016/j.ijbiomac.2020.10.238., Epub 2020 Nov 2. PMID:33152360<ref>PMID:33152360</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bov" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:28, 20 January 2021
The Structure of Bacillus subtilis glycosyltransferase,Bs-YjiC
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Categories: Bacsu | Large Structures | Bao, R | Liu, B | Luo, Y Z | Zhao, C | Zhao, N L | Glycosyltransferase | Transferase
