Sandbox Reserved 1628

Adhesins, commonly found on the surface of infections organisms, aid in binding or adhering the pathogen to other cells. This is normally the beginning of an infection as the pathogen can bind to a host cell and potentially start a cascade of events within the host cell that leads to an infection^[1]. Various pathogens have various types of adhesin proteins, but this page looks specifically at epithelial adhesins, particularly one found in Candida glabrata, a fungi which causes infections most often in immunosuppressed patients^[2] and can lead to deadly bloodstream infections.

Function of your Protein

Candida glabrata is a fungal organism that commonly interacts with other similar fungi such as Candida albicans to infect humans with what are commonly known as thrush, yeast infections, and urinary tract infections^[2]. The fungi is a type of yeast which grows on the human body naturally, it's only once it enters the body somehow that it starts to cause infections. For the pathogen to do this, it must first adhere to a host cell and does so by using the adhesins found on the outside of the fungi.

EpaA1 looks specifically for certain sugars on the outside of the host cells and will bind to those. binds to beta-D-galactopyranose-(1,3)-beta-D-glucopyranose. For Candida glabrata, there are about 20 different types of epithelial adhesins, each with conserved areas of the binding site and variable areas to be able to bind to a variety of sugars on the host cell^[3].

//The protein is mainly made of antiparallel beta sheets, with the loops connecting the sheets making up the most critical parts of the active site. Two loops responsible for binding to the calcium ion, CBL1 and CLB2, are closer to the rest of the protein, and loops L1-L3 are further out - some of these loops are highly conserved between different adhesins, some are very variable.//

Biological relevance and broader implications

For Candida glabrata to infect host cells, it must first attach itself to said cell. By having the active site within the protein have both conserved and variable areas, it allows the fungal pathogen to have a wider array of hosts it can bind to. This also leads to it being resistant for various treatments commonly used for similar fungi and can potentially enter and infect the bloodstream of a patient.

Current research is investigating which sugars the binding site works best with, what parts of the binding site they interact with, and how potential treatments can be made from these findings. By determining which pieces of the binding site are conserved, new drugs can be produced that can interact with those areas and prevent the adhesin from binding to an actual host cell. This is especially necessary for those who are immunocompromised and may be at a higher risk of not only becoming infected with the fungal pathogen, but dying from the resulting bloodstream infection.

Important amino acids

The ligand for this specific EpaA1 is the sugar . To keep the sugar bound, within the active site there are 4 amino acids which hydrogen bind to it - . The Arg and Asp amino acids are very conserved between the various epithelial adhesin active sites and are considered critical for effective binding whereas the Glu is more variable and specific to binding this sugar.

Structural highlights

Other important features

Tryptophan causing hydrophobic patch to keep sugar in place. Changing it to another w/aromatic ring didn't change binding much, hydrophobic aromatic ring more important. Calcium ion, many hydrogen bonds between the sugar and the ion - very necessary.