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| | <StructureSection load='1p4p' size='340' side='right'caption='[[1p4p]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='1p4p' size='340' side='right'caption='[[1p4p]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1p4p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35210 Atcc 35210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1P4P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1p4p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P4P FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1osp|1osp]], [[1fj1|1fj1]], [[1ggq|1ggq]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OSPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=139 ATCC 35210])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4p OCA], [https://pdbe.org/1p4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p4p RCSB], [https://www.ebi.ac.uk/pdbsum/1p4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p4p ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1p4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4p OCA], [http://pdbe.org/1p4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p4p RCSB], [http://www.ebi.ac.uk/pdbsum/1p4p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1p4p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/OSPB_BORBU OSPB_BORBU] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[G14secL04Tpc3|G14secL04Tpc3]] | |
| | *[[Outer surface protein|Outer surface protein]] | | *[[Outer surface protein|Outer surface protein]] |
| | == References == | | == References == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 35210]] | + | [[Category: Borreliella burgdorferi]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Barbour, A G]] | + | [[Category: Barbour AG]] |
| - | [[Category: Becker, M]] | + | [[Category: Becker M]] |
| - | [[Category: Bunikis, J]] | + | [[Category: Bunikis J]] |
| - | [[Category: Dunn, J J]] | + | [[Category: Dunn JJ]] |
| - | [[Category: Lade, B D]] | + | [[Category: Lade BD]] |
| - | [[Category: Lawson, C L]] | + | [[Category: Lawson CL]] |
| - | [[Category: Extended beta sheet]]
| + | |
| - | [[Category: Intermolecular beta sheet]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
OSPB_BORBU
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Certain antibody Fab fragments directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi, have the unusual property of being bactericidal even in the absence of complement. We report here x-ray crystal structures of a C-terminal fragment of B. burgdorferi OspB, which spans residues 152-296, alone at 2.0-A resolution, and in a complex with the bactericidal Fab H6831 at 2.6-A resolution. The H6831 epitope is topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for H6831 recognition. A beta-sheet present in the free OspB fragment is either disordered or removed by proteolysis in the H6831-bound complex. Other conformational changes between free and H6831-bound structures are minor and appear to be related to this loss. In both crystal structures, OspB C-terminal fragments form artificial dimers connected by intermolecular beta-sheets. OspB structure, stability, and possible mechanisms of killing by H6831 and other bactericidal Fabs are discussed in light of the structural data.
Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target.,Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL. Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target. J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683 doi:10.1074/jbc.M412842200
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