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| | <StructureSection load='6yfc' size='340' side='right'caption='[[6yfc]], [[Resolution|resolution]] 3.25Å' scene=''> | | <StructureSection load='6yfc' size='340' side='right'caption='[[6yfc]], [[Resolution|resolution]] 3.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6yfc]] is a 180 chain structure with sequence from [http://en.wikipedia.org/wiki/Leviviridae_sp. Leviviridae sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YFC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YFC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yfc]] is a 180 chain structure with sequence from [https://en.wikipedia.org/wiki/Leviviridae_sp. Leviviridae sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YFC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.246Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6yf7|6yf7]], [[6yf9|6yf9]], [[6yfa|6yfa]], [[6yfb|6yfb]], [[6yfd|6yfd]], [[6yfe|6yfe]], [[6yff|6yff]], [[6yfg|6yfg]], [[6yfh|6yfh]], [[6yfi|6yfi]], [[6yfj|6yfj]], [[6yfk|6yfk]], [[6yfl|6yfl]], [[6yfm|6yfm]], [[6yfn|6yfn]], [[6yfo|6yfo]], [[6yfp|6yfp]], [[6yfq|6yfq]], [[6yfr|6yfr]], [[6yfs|6yfs]], [[6yft|6yft]], [[6yfu|6yfu]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yfc OCA], [http://pdbe.org/6yfc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yfc RCSB], [http://www.ebi.ac.uk/pdbsum/6yfc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yfc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yfc OCA], [https://pdbe.org/6yfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yfc RCSB], [https://www.ebi.ac.uk/pdbsum/6yfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yfc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Leviviridae sp]] | | [[Category: Leviviridae sp]] |
| - | [[Category: Kalnins, G]] | + | [[Category: Kalnins G]] |
| - | [[Category: Lieknina, I]] | + | [[Category: Lieknina I]] |
| - | [[Category: Rumnieks, J]] | + | [[Category: Rumnieks J]] |
| - | [[Category: Sisovs, M]] | + | [[Category: Sisovs M]] |
| - | [[Category: Tars, K]] | + | [[Category: Tars K]] |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Virus]]
| + | |
| - | [[Category: Virus like particle]]
| + | |
| - | [[Category: Virus-like particle]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The single-stranded RNA (ssRNA) bacteriophages are among the simplest known viruses with small genomes and exceptionally high mutation rates. The number of ssRNA phage isolates has remained very low, but recent metagenomic studies have uncovered an immense variety of distinct uncultured ssRNA phages. The coat proteins (CPs) in these genomes are particularly diverse, with notable variation in length and often no recognizable similarity to previously known viruses. We recombinantly expressed metagenome-derived ssRNA phage CPs to produce virus-like particles and determined the three-dimensional structure of 22 previously uncharacterized ssRNA phage capsids covering nine distinct CP types. The structures revealed substantial deviations from the previously known ssRNA phage CP fold, uncovered an unusual prolate particle shape, and revealed a previously unseen dsRNA binding mode. These data expand our knowledge of the evolution of viral structural proteins and are of relevance for applications such as ssRNA phage-based vaccine design.
Three-dimensional structure of 22 uncultured ssRNA bacteriophages: Flexibility of the coat protein fold and variations in particle shapes.,Rumnieks J, Lieknina I, Kalnins G, Sisovs M, Akopjana I, Bogans J, Tars K Sci Adv. 2020 Sep 2;6(36). pii: 6/36/eabc0023. doi: 10.1126/sciadv.abc0023. Print, 2020 Sep. PMID:32917600[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rumnieks J, Lieknina I, Kalnins G, Sisovs M, Akopjana I, Bogans J, Tars K. Three-dimensional structure of 22 uncultured ssRNA bacteriophages: Flexibility of the coat protein fold and variations in particle shapes. Sci Adv. 2020 Sep 2;6(36). pii: 6/36/eabc0023. doi: 10.1126/sciadv.abc0023. Print, 2020 Sep. PMID:32917600 doi:http://dx.doi.org/10.1126/sciadv.abc0023
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