1qoy

From Proteopedia

(Difference between revisions)

Revision as of 08:05, 21 September 2022

E.coli Hemolysin E (HlyE, ClyA, SheA)

Structural highlights

1qoy is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HLYE_ECOLI] Toxin, which has some hemolytic activity towards mammalian cells. Acts by forming a pore-like structure upon contact with mammalian cells.^[1]

Publication Abstract from PubMed

Hemolysin E (HlyE) is a novel pore-forming toxin of Escherichia coli, Salmonella typhi, and Shigella flexneri. Here we report the X-ray crystal structure of the water-soluble form of E. coli HlyE at 2.0 A resolution and the visualization of the lipid-associated form of the toxin in projection at low resolution by electron microscopy. The crystal structure reveals HlyE to be the first member of a new family of toxin structures, consisting of an elaborated helical bundle some 100 A long. The electron micrographs show how HlyE oligomerizes in the presence of lipid to form transmembrane pores. Taken together, the data from these two structural techniques allow us to propose a simple model for the structure of the pore and for membrane interaction.

E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy.,Wallace AJ, Stillman TJ, Atkins A, Jamieson SJ, Bullough PA, Green J, Artymiuk PJ Cell. 2000 Jan 21;100(2):265-76. PMID:10660049^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wai SN, Lindmark B, Soderblom T, Takade A, Westermark M, Oscarsson J, Jass J, Richter-Dahlfors A, Mizunoe Y, Uhlin BE. Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin. Cell. 2003 Oct 3;115(1):25-35. PMID:14532000
↑ Wallace AJ, Stillman TJ, Atkins A, Jamieson SJ, Bullough PA, Green J, Artymiuk PJ. E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy. Cell. 2000 Jan 21;100(2):265-76. PMID:10660049

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qoy"

@@ Line 3: / Line 3: @@
 <StructureSection load='1qoy' size='340' side='right'caption='[[1qoy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1qoy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QOY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1QOY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1qoy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QOY FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HLYE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qoy OCA], [https://pdbe.org/1qoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qoy RCSB], [https://www.ebi.ac.uk/pdbsum/1qoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qoy ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1qoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qoy OCA], [http://pdbe.org/1qoy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qoy RCSB], [http://www.ebi.ac.uk/pdbsum/1qoy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qoy ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[https://www.uniprot.org/uniprot/HLYE_ECOLI HLYE_ECOLI]] Toxin, which has some hemolytic activity towards mammalian cells. Acts by forming a pore-like structure upon contact with mammalian cells.<ref>PMID:14532000</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 ==See Also==
 *[[Hemolysin 3D structures|Hemolysin 3D structures]]
-*[[Molecular Playground/ClyA|Molecular Playground/ClyA]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Artymiuk, P J]]
+[[Category: Artymiuk PJ]]
-[[Category: Atkins, A]]
+[[Category: Atkins A]]
-[[Category: Bullough, P A]]
+[[Category: Bullough PA]]
-[[Category: Green, J]]
+[[Category: Green J]]
-[[Category: Jamieson, S J]]
+[[Category: Jamieson SJ]]
-[[Category: Stillman, T J]]
+[[Category: Stillman TJ]]
-[[Category: Wallace, A J]]
+[[Category: Wallace AJ]]
-[[Category: Cytolysin]]
-[[Category: Hemolysin]]
-[[Category: Membrane pore former]]
-[[Category: Pore]]
-[[Category: Toxin]]

1qoy

From Proteopedia

Revision as of 08:05, 21 September 2022

E.coli Hemolysin E (HlyE, ClyA, SheA)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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