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| - | [[Image:1dba.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1dba.png|left|200px]] |
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| | {{STRUCTURE_1dba| PDB=1dba | SCENE= }} | | {{STRUCTURE_1dba| PDB=1dba | SCENE= }} |
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| - | '''THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX'''
| + | ===THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX=== |
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| - | ==Overview==
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| - | The monoclonal anti-progesterone antibody DB3 binds progesterone with nanomolar affinity (Ka approximately 10(9) M-1), suggesting high specificity. However, DB3 also cross-reacts with similar affinity with a subgroup of structurally distinct, progesterone-like steroids. Crystals of the unliganded Fab' and various steroid-Fab' complexes are isomorphous and belong to the hexagonal space group, P6(4)22, with unit cell dimensions of a = b = 135 A, c = 124 A. Structures of free and progesterone-bound Fab' have been determined by X-ray crystallography at 2.7 A resolution using molecular replacement techniques. Progesterone is bound in a hydrophobic pocket formed mainly by the interaction of three complementarity determining regions L1, H2 and H3. The orientation of the ligand in the binding site was aided by both crystallographic and biochemical analyses of substituted steroids. The indole side-chain of TrpH100 of the DB3 has two different conformations, inter-converting "open" and "closed" forms of the antibody combining site. The TrpH100 indole thus appears to be acting as an antibody-derived surrogate ligand for its own hydrophobic binding pocket. These structures provide the first atomic view of how a steroid interacts with a protein and offer a structural explanation for the restriction of the anti-progesterone response to the VGAM3.8 family of VH genes. | + | The line below this paragraph, {{ABSTRACT_PUBMED_8496956}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8496956 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8496956}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Wilson, I A.]] | | [[Category: Wilson, I A.]] |
| | [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:39:17 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:46:27 2008'' |
Revision as of 19:46, 30 June 2008
Template:STRUCTURE 1dba
THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX
Template:ABSTRACT PUBMED 8496956
About this Structure
1DBA is a Protein complex structure. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of an anti-steroid Fab' and progesterone-Fab' complex., Arevalo JH, Stura EA, Taussig MJ, Wilson IA, J Mol Biol. 1993 May 5;231(1):103-18. PMID:8496956
Page seeded by OCA on Mon Jun 30 22:46:27 2008
