1rcv

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(Difference between revisions)

Revision as of 08:05, 21 September 2022

Cholera Toxin B-Pentamer Complexed With Bivalent Nitrophenol-Galactoside Ligand BV1

Structural highlights

1rcv is a 5 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CHTB_VIBCH] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Publication Abstract from PubMed

A series of bivalent ligands of varying length were synthesized to inhibit the receptor-binding process of cholera toxin. Competitive surface receptor binding assays showed that significant potency gains relative to the constituent monovalent ligands were achieved independently from the ability of the extended bivalent ligands to span binding sites within the toxin pentamer. Several models that could account for the unexpected improvement in IC(50) values are examined, taking into account crystallographic analysis of each ligand in complex with the toxin pentamer. Evidence is presented that steric blocking at the receptor binding surface may play a role. The results of our study suggest that the use of relatively short, "nonspanning" bivalent ligands, or monovalent ligands of similar topology and bulk may be an effective way of blocking the interaction of multimeric proteins with their cell surface receptors.

Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer.,Pickens JC, Mitchell DD, Liu J, Tan X, Zhang Z, Verlinde CL, Hol WG, Fan E Chem Biol. 2004 Sep;11(9):1205-15. PMID:15380181^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pickens JC, Mitchell DD, Liu J, Tan X, Zhang Z, Verlinde CL, Hol WG, Fan E. Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer. Chem Biol. 2004 Sep;11(9):1205-15. PMID:15380181 doi:10.1016/j.chembiol.2004.06.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rcv"

@@ Line 3: / Line 3: @@
 <StructureSection load='1rcv' size='340' side='right'caption='[[1rcv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1rcv]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RCV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1rcv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCV FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BV1:[3-(4-{3-[3-NITRO-5-(GALACTOPYRANOSYLOXY)-BENZOYLAMINO]-PROPYL}-PIPERAZIN-1-YL)-PROPYLAMINO]+-2-(3-{4-[3-(3-NITRO-5-[GALACTOPYRANOSYLOXY]-BENZOYLAMINO)-PROPYL]-PIPERAZIN-1-YL}+-PROPYL-AMINO)-3,4-DIOXO-CYCLOBUTENE'>BV1</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rd9|1rd9]], [[1rdp|1rdp]], [[1rf2|1rf2]]</div></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcv OCA], [https://pdbe.org/1rcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcv RCSB], [https://www.ebi.ac.uk/pdbsum/1rcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcv ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ctxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcv OCA], [http://pdbe.org/1rcv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rcv RCSB], [http://www.ebi.ac.uk/pdbsum/1rcv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcv ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
+[[https://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 28: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillo virgola del koch trevisan 1884]]
 [[Category: Large Structures]]
-[[Category: Fan, E]]
+[[Category: Vibrio cholerae]]
-[[Category: Hol, W G]]
+[[Category: Fan E]]
-[[Category: Liu, J]]
+[[Category: Hol WG]]
-[[Category: Mitchell, D D]]
+[[Category: Liu J]]
-[[Category: Pickens, J C]]
+[[Category: Mitchell DD]]
-[[Category: Tan, X]]
+[[Category: Pickens JC]]
-[[Category: Verlinde, C L]]
+[[Category: Tan X]]
-[[Category: Zhang, Z]]
+[[Category: Verlinde CL]]
-[[Category: Bivalent]]
+[[Category: Zhang Z]]
-[[Category: Cholera]]
-[[Category: Complex]]
-[[Category: Pentamer]]
-[[Category: Toxin]]

1rcv

From Proteopedia

Revision as of 08:05, 21 September 2022

Cholera Toxin B-Pentamer Complexed With Bivalent Nitrophenol-Galactoside Ligand BV1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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