6ve4
From Proteopedia
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==Pentadecameric PilQ from Pseudomonas aeruginosa== | ==Pentadecameric PilQ from Pseudomonas aeruginosa== | ||
| - | <StructureSection load='6ve4' size='340' side='right'caption='[[6ve4]]' scene=''> | + | <StructureSection load='6ve4' size='340' side='right'caption='[[6ve4]], [[Resolution|resolution]] 6.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VE4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VE4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ve4]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VE4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VE4 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ve4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ve4 OCA], [http://pdbe.org/6ve4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ve4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ve4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ve4 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ve4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ve4 OCA], [http://pdbe.org/6ve4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ve4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ve4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ve4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/PILQ_PSEAE PILQ_PSEAE]] Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a 'porthole' allowing passage of the fimbrae through the outer membrane. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The type IV pilus machinery is a multi-protein complex that polymerizes and depolymerizes a pilus fiber used for attachment, twitching motility, phage adsorption, natural competence, protein secretion, and surface-sensing. An outer membrane secretin pore is required for passage of the pilus fiber out of the cell. Herein, the structure of the tetradecameric secretin, PilQ, from the Pseudomonas aeruginosa type IVa pilus system was determined to 4.3 A and 4.4 A resolution in the presence and absence of C7 symmetric spikes, respectively. The heptameric spikes were found to be two tandem C-terminal domains of TsaP. TsaP forms a belt around PilQ and while it is not essential for twitching motility, overexpression of TsaP triggers a signal cascade upstream of PilY1 leading to cyclic di-GMP up-regulation. These results resolve the identity of the spikes identified with Proteobacterial PilQ homologs and may reveal a new component of the surface-sensing cyclic di-GMP signal cascade. | ||
| + | |||
| + | CryoEM map of Pseudomonas aeruginosa PilQ enables structural characterization of TsaP.,McCallum M, Tammam S, Rubinstein JL, Burrows LL, Howell PL Structure. 2020 Dec 11. pii: S0969-2126(20)30466-4. doi:, 10.1016/j.str.2020.11.019. PMID:33338410<ref>PMID:33338410</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ve4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Burrows | + | [[Category: Burrows, L L]] |
| - | [[Category: Howell | + | [[Category: Howell, P L]] |
| - | [[Category: McCallum M]] | + | [[Category: McCallum, M]] |
| - | [[Category: Rubinstein | + | [[Category: Rubinstein, J L]] |
| - | [[Category: Tammam S]] | + | [[Category: Tammam, S]] |
| + | [[Category: Bacterial secretion system]] | ||
| + | [[Category: Outer membrane]] | ||
| + | [[Category: Periplasm]] | ||
| + | [[Category: Pilq]] | ||
| + | [[Category: Pilus]] | ||
| + | [[Category: Protein transport]] | ||
| + | [[Category: Secretin]] | ||
| + | [[Category: T4ap]] | ||
| + | [[Category: T4p]] | ||
| + | [[Category: Type iv pilus]] | ||
| + | [[Category: Type iva pilus]] | ||
Revision as of 06:57, 30 December 2020
Pentadecameric PilQ from Pseudomonas aeruginosa
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