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| | <StructureSection load='1gnt' size='340' side='right'caption='[[1gnt]], [[Resolution|resolution]] 1.25Å' scene=''> | | <StructureSection load='1gnt' size='340' side='right'caption='[[1gnt]], [[Resolution|resolution]] 1.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1gnt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29579 Atcc 29579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1GNT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1gnt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GNT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FSO:IRON/SULFUR/OXYGEN+HYBRID+CLUSTER'>FSO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e1d|1e1d]], [[1e2u|1e2u]], [[1e9v|1e9v]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FSO:IRON/SULFUR/OXYGEN+HYBRID+CLUSTER'>FSO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gnt OCA], [http://pdbe.org/1gnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gnt RCSB], [http://www.ebi.ac.uk/pdbsum/1gnt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gnt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gnt OCA], [https://pdbe.org/1gnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gnt RCSB], [https://www.ebi.ac.uk/pdbsum/1gnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gnt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HCP_DESVH HCP_DESVH]] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity). | + | [https://www.uniprot.org/uniprot/HCP_DESVH HCP_DESVH] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 29579]] | + | [[Category: Desulfovibrio vulgaris]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bailey, S]] | + | [[Category: Bailey S]] |
| - | [[Category: Carrondo, M A]] | + | [[Category: Carrondo MA]] |
| - | [[Category: Coelho, R]] | + | [[Category: Coelho R]] |
| - | [[Category: Cooper, S J]] | + | [[Category: Cooper SJ]] |
| - | [[Category: Garner, D C]] | + | [[Category: Garner DC]] |
| - | [[Category: Hagen, W R]] | + | [[Category: Hagen WR]] |
| - | [[Category: Legall, J]] | + | [[Category: Legall J]] |
| - | [[Category: Lindley, P]] | + | [[Category: Lindley P]] |
| - | [[Category: Liu, M Y]] | + | [[Category: Liu MY]] |
| - | [[Category: Macedo, S]] | + | [[Category: Macedo S]] |
| - | [[Category: Mitchell, E P]] | + | [[Category: Mitchell EP]] |
| - | [[Category: Romao, C V]] | + | [[Category: Romao CV]] |
| - | [[Category: Teixeira, M]] | + | [[Category: Teixeira M]] |
| - | [[Category: Xavier, A V]] | + | [[Category: Xavier AV]] |
| - | [[Category: Aerobic desulfovibrio vulgari]]
| + | |
| - | [[Category: Hybrid cluster protein]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
HCP_DESVH Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structures of the hybrid cluster proteins (HCPs) from the sulfate-reducing bacteria Desulfovibrio desulfuricans (ATCC 27774) and Desulfovibrio vulgaris (Hildenborough) have been elucidated at a resolution of 1.25 A using X-ray synchrotron radiation techniques. In the case of the D. desulfuricans protein, protein isolation, purification, crystallization and X-ray data collection were carried out under strict anaerobic conditions, whereas for the D. vulgaris protein the conditions were aerobic. However, both structures are essentially the same, comprising three domains and two iron-sulfur centres. One of these centres situated near the exterior of the molecules in domain 1 is a cubane [4Fe-4S] cluster, whereas the other, located at the interface of the three domains, contains the unusual four-iron cluster initially found in the D. vulgaris protein. Details of the structures and the associated EPR spectroscopy of the D. desulfuricans protein are reported herein. These structures show that the nature of the hybrid cluster, containing both oxygen and sulfur bridges, is independent of the presence of oxygen in the isolation and crystallization procedure and also does not vary significantly with changes in the oxidation state. The structures and amino acid sequences of the HCP are compared with the recently elucidated structure of the catalytic subunit of a carbon monoxide dehydrogenase from Carboxydothermus hydrogenoformans and related dehydrogenases. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0326-y.
Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 A resolution using synchrotron radiation.,Macedo S, Mitchell EP, Romao CV, Cooper SJ, Coelho R, Liu MY, Xavier AV, LeGall J, Bailey S, Garner DC, Hagen WR, Teixeira M, Carrondo MA, Lindley P J Biol Inorg Chem. 2002 Apr;7(4-5):514-25. Epub 2002 Jan 23. PMID:11941509[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Macedo S, Mitchell EP, Romao CV, Cooper SJ, Coelho R, Liu MY, Xavier AV, LeGall J, Bailey S, Garner DC, Hagen WR, Teixeira M, Carrondo MA, Lindley P. Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 A resolution using synchrotron radiation. J Biol Inorg Chem. 2002 Apr;7(4-5):514-25. Epub 2002 Jan 23. PMID:11941509 doi:10.1007/s00775-001-0326-y
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