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| | ==NMR solution structure of class IV lasso peptide felipeptin A1 from Amycolatopsis sp. YIM10== | | ==NMR solution structure of class IV lasso peptide felipeptin A1 from Amycolatopsis sp. YIM10== |
| - | <StructureSection load='6xth' size='340' side='right'caption='[[6xth]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6xth' size='340' side='right'caption='[[6xth]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6xth]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amycolatopsis_sp. Amycolatopsis sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XTH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XTH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xth]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_sp. Amycolatopsis sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XTH FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6xti|6xti]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xth OCA], [https://pdbe.org/6xth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xth RCSB], [https://www.ebi.ac.uk/pdbsum/6xth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xth ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xth OCA], [http://pdbe.org/6xth PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xth RCSB], [http://www.ebi.ac.uk/pdbsum/6xth PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xth ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A5P9PRQ2_9PSEU A0A5P9PRQ2_9PSEU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Amycolatopsis sp]] | | [[Category: Amycolatopsis sp]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aachmann, F L]] | + | [[Category: Aachmann FL]] |
| - | [[Category: Courtade, G]] | + | [[Category: Courtade G]] |
| - | [[Category: Guerrero-Garzon, J F]] | + | [[Category: Guerrero-Garzon JF]] |
| - | [[Category: Madland, E]] | + | [[Category: Madland E]] |
| - | [[Category: Zotchev, S B]] | + | [[Category: Zotchev SB]] |
| - | [[Category: Antibacterial]]
| + | |
| - | [[Category: Class iv]]
| + | |
| - | [[Category: Lasso peptide]]
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| - | [[Category: Unknown function]]
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| Structural highlights
Function
A0A5P9PRQ2_9PSEU
Publication Abstract from PubMed
Heterologous expression of a biosynthesis gene cluster from Amycolatopsis sp. resulted in the discovery of two unique class IV lasso peptides, felipeptins A1 and A2. A mixture of felipeptins stimulated proliferation of cancer cells, while having no such effect on the normal cells. Detailed investigation revealed, that pre-treatment of cancer cells with a mixture of felipeptins resulted in downregulation of the tumor suppressor Rb, making the cancer cells to proliferate faster. Pre-treatment with felipeptins made cancer cells considerably more sensitive to the anticancer agent doxorubicin and re-sensitized doxorubicin-resistant cells to this drug. Structural characterization and binding experiments showed an interaction between felipeptins resulting in complex formation, which explains their synergistic effect. This discovery may open an alternative avenue in cancer treatment, helping to eliminate quiescent cells that often lead to cancer relapse.
Class IV Lasso Peptides Synergistically Induce Proliferation of Cancer Cells and Sensitize Them to Doxorubicin.,Guerrero-Garzon JF, Madland E, Zehl M, Singh M, Rezaei S, Aachmann FL, Courtade G, Urban E, Ruckert C, Busche T, Kalinowski J, Cao YR, Jiang Y, Jiang CL, Selivanova G, Zotchev SB iScience. 2020 Nov 10;23(12):101785. doi: 10.1016/j.isci.2020.101785. eCollection, 2020 Dec 18. PMID:33294793[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guerrero-Garzon JF, Madland E, Zehl M, Singh M, Rezaei S, Aachmann FL, Courtade G, Urban E, Ruckert C, Busche T, Kalinowski J, Cao YR, Jiang Y, Jiang CL, Selivanova G, Zotchev SB. Class IV Lasso Peptides Synergistically Induce Proliferation of Cancer Cells and Sensitize Them to Doxorubicin. iScience. 2020 Nov 10;23(12):101785. doi: 10.1016/j.isci.2020.101785. eCollection, 2020 Dec 18. PMID:33294793 doi:http://dx.doi.org/10.1016/j.isci.2020.101785
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