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| | <StructureSection load='1u0u' size='340' side='right'caption='[[1u0u]], [[Resolution|resolution]] 2.11Å' scene=''> | | <StructureSection load='1u0u' size='340' side='right'caption='[[1u0u]], [[Resolution|resolution]] 2.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1u0u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pinsy Pinsy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1U0U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1u0u]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pinus_sylvestris Pinus sylvestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0U FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bi5|1bi5]], [[1u0v|1u0v]], [[1u0w|1u0w]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PSS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3349 PINSY])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0u OCA], [https://pdbe.org/1u0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0u RCSB], [https://www.ebi.ac.uk/pdbsum/1u0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0u ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1u0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0u OCA], [http://pdbe.org/1u0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0u RCSB], [http://www.ebi.ac.uk/pdbsum/1u0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0u ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DPSS_PINSY DPSS_PINSY] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pinsy]] | + | [[Category: Pinus sylvestris]] |
| - | [[Category: Austin, M B]] | + | [[Category: Austin MB]] |
| - | [[Category: Bowman, M E]] | + | [[Category: Bowman ME]] |
| - | [[Category: Ferrer, J L]] | + | [[Category: Ferrer J-L]] |
| - | [[Category: Noel, J P]] | + | [[Category: Noel JP]] |
| - | [[Category: Schroder, J]] | + | [[Category: Schroder J]] |
| - | [[Category: Aldol switch]]
| + | |
| - | [[Category: Alpha-beta-alpha-beta-alpha fold]]
| + | |
| - | [[Category: Catalytic triad]]
| + | |
| - | [[Category: Condensing enzyme]]
| + | |
| - | [[Category: Pk]]
| + | |
| - | [[Category: Thiolase fold]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Type iii polyketide synthase]]
| + | |
| Structural highlights
Function
DPSS_PINSY
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Stilbene synthase (STS) and chalcone synthase (CHS) each catalyze the formation of a tetraketide intermediate from a CoA-tethered phenylpropanoid starter and three molecules of malonyl-CoA, but use different cyclization mechanisms to produce distinct chemical scaffolds for a variety of plant natural products. Here we present the first STS crystal structure and identify, by mutagenic conversion of alfalfa CHS into a functional stilbene synthase, the structural basis for the evolution of STS cyclization specificity in type III polyketide synthase (PKS) enzymes. Additional mutagenesis and enzymatic characterization confirms that electronic effects rather than steric factors balance competing cyclization specificities in CHS and STS. Finally, we discuss the problematic in vitro reconstitution of plant stilbenecarboxylate pathways, using insights from existing biomimetic polyketide cyclization studies to generate a novel mechanistic hypothesis to explain stilbenecarboxylate biosynthesis.
An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases.,Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP Chem Biol. 2004 Sep;11(9):1179-94. PMID:15380179[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP. An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases. Chem Biol. 2004 Sep;11(9):1179-94. PMID:15380179 doi:http://dx.doi.org/10.1016/j.chembiol.2004.05.024
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