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| | <StructureSection load='1u1x' size='340' side='right'caption='[[1u1x]], [[Resolution|resolution]] 1.88Å' scene=''> | | <StructureSection load='1u1x' size='340' side='right'caption='[[1u1x]], [[Resolution|resolution]] 1.88Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1u1x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U1X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1U1X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1u1x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U1X FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HHA:(2S,3S)-TRANS-2,3-DIHYDRO-3-HYDROXYANTHRANILIC+ACID'>HHA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bwz|1bwz]], [[1sdj|1sdj]], [[1s7j|1s7j]], [[1u1v|1u1v]], [[1u1w|1u1w]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HHA:(2S,3S)-TRANS-2,3-DIHYDRO-3-HYDROXYANTHRANILIC+ACID'>HHA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phzF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u1x OCA], [https://pdbe.org/1u1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u1x RCSB], [https://www.ebi.ac.uk/pdbsum/1u1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u1x ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1u1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u1x OCA], [http://pdbe.org/1u1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u1x RCSB], [http://www.ebi.ac.uk/pdbsum/1u1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u1x ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PHZF_PSEFL PHZF_PSEFL]] Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known.<ref>PMID:19053436</ref> <ref>PMID:15545603</ref> | + | [https://www.uniprot.org/uniprot/PHZF_PSEFL PHZF_PSEFL] Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known.<ref>PMID:19053436</ref> <ref>PMID:15545603</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus fluorescens liquefaciens flugge 1886]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bayer, P]] | + | [[Category: Pseudomonas fluorescens]] |
| - | [[Category: Blankenfeldt, W]] | + | [[Category: Bayer P]] |
| - | [[Category: Janning, P]] | + | [[Category: Blankenfeldt W]] |
| - | [[Category: Korniyenko, Y]] | + | [[Category: Janning P]] |
| - | [[Category: Kuzin, A P]] | + | [[Category: Korniyenko Y]] |
| - | [[Category: Mavrodi, D V]] | + | [[Category: Kuzin AP]] |
| - | [[Category: Skarina, T]] | + | [[Category: Mavrodi DV]] |
| - | [[Category: Thomashow, L S]] | + | [[Category: Skarina T]] |
| - | [[Category: Tong, L]] | + | [[Category: Thomashow LS]] |
| - | [[Category: Acid/base catalysis]]
| + | [[Category: Tong L]] |
| - | [[Category: Closed form]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Phenazine biosynthesis]]
| + | |
| - | [[Category: Substrate complex]]
| + | |
| Structural highlights
Function
PHZF_PSEFL Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic nitrogen-containing metabolites with antibiotic, antitumor, and antiparasitic activity. The antibiotic properties of pyocyanin, produced by Pseudomonas aeruginosa, were recognized in the 1890s, although this blue phenazine is now known to be a virulence factor in human disease. Despite their biological significance, the biosynthesis of phenazines is not fully understood. Here we present structural and functional studies of PhzF, an enzyme essential for phenazine synthesis in Pseudomonas spp. PhzF shares topology with diaminopimelate epimerase DapF but lacks the same catalytic residues. The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an isomerase using the conserved glutamate E45 to abstract a proton from C3 of the substrate. The proton is returned to C1 of the substrate after rearrangement of the double-bond system, yielding an enol that converts to the corresponding ketone. PhzF is a dimer that may be bifunctional, providing a shielded cavity for ketone dimerization via double Schiff-base formation to produce the phenazine scaffold. Our proposed mechanism is supported by mass and NMR spectroscopy. The results are discussed in the context of related structures and protein sequences of unknown biochemical function.
Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens.,Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ahuja EG, Janning P, Mentel M, Graebsch A, Breinbauer R, Hiller W, Costisella B, Thomashow LS, Mavrodi DV, Blankenfeldt W. PhzA/B catalyzes the formation of the tricycle in phenazine biosynthesis. J Am Chem Soc. 2008 Dec 17;130(50):17053-61. PMID:19053436 doi:10.1021/ja806325k
- ↑ Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV. Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens. Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603
- ↑ Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV. Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens. Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603
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