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1wij

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Revision as of 06:56, 2 March 2022

Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3

Structural highlights

1wij is a 1 chain structure with sequence from Arath. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	EIL3 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[EIL3_ARATH] Probable transcription factor that may be involved in the ethylene response pathway.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The EIN3/EIL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy. The structure consists of five alpha-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding.

Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3.,Yamasaki K, Kigawa T, Inoue M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S J Mol Biol. 2005 Apr 29;348(2):253-64. PMID:15811366^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker JR. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell. 1997 Jun 27;89(7):1133-44. PMID:9215635
↑ Solano R, Stepanova A, Chao Q, Ecker JR. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev. 1998 Dec 1;12(23):3703-14. PMID:9851977
↑ Yamasaki K, Kigawa T, Inoue M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S. Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3. J Mol Biol. 2005 Apr 29;348(2):253-64. PMID:15811366 doi:10.1016/j.jmb.2005.02.065

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wij"

@@ Line 3: / Line 3: @@
 <StructureSection load='1wij' size='340' side='right'caption='[[1wij]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1wij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1WIJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1wij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WIJ FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EIL3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EIL3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1wij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wij OCA], [http://pdbe.org/1wij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wij RCSB], [http://www.ebi.ac.uk/pdbsum/1wij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wij ProSAT], [http://www.topsan.org/Proteins/RSGI/1wij TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wij OCA], [https://pdbe.org/1wij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wij RCSB], [https://www.ebi.ac.uk/pdbsum/1wij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wij ProSAT], [https://www.topsan.org/Proteins/RSGI/1wij TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/EIL3_ARATH EIL3_ARATH]] Probable transcription factor that may be involved in the ethylene response pathway.<ref>PMID:9215635</ref> <ref>PMID:9851977</ref>
+[[https://www.uniprot.org/uniprot/EIL3_ARATH EIL3_ARATH]] Probable transcription factor that may be involved in the ethylene response pathway.<ref>PMID:9215635</ref> <ref>PMID:9851977</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1wij

From Proteopedia

Revision as of 06:56, 2 March 2022

Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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