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| | <StructureSection load='1xns' size='340' side='right'caption='[[1xns]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='1xns' size='340' side='right'caption='[[1xns]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1xns]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp1 Bpp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XNS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1xns]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P1 Escherichia virus P1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XNS FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3crx|3crx]], [[1xo0|1xo0]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CRE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10678 BPP1])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xns OCA], [https://pdbe.org/1xns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xns RCSB], [https://www.ebi.ac.uk/pdbsum/1xns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xns ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xns OCA], [http://pdbe.org/1xns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xns RCSB], [http://www.ebi.ac.uk/pdbsum/1xns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xns ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1]] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. | + | [https://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpp1]] | + | [[Category: Escherichia virus P1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Duyne, G D.Van]]
| + | [[Category: Ghosh K]] |
| - | [[Category: Ghosh, K]] | + | [[Category: Guo F]] |
| - | [[Category: Guo, F]] | + | [[Category: Lau CK]] |
| - | [[Category: Lau, C K]] | + | [[Category: Segall AM]] |
| - | [[Category: Segall, A M]] | + | [[Category: Van Duyne GD]] |
| - | [[Category: Cre recombinase]] | + | |
| - | [[Category: Holliday junction]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Ligase-dna complex]]
| + | |
| - | [[Category: Peptide inhibitor]]
| + | |
| - | [[Category: Recombination]]
| + | |
| Structural highlights
Function
RECR_BPP1 Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Cre recombinase is a prototypical member of the tyrosine recombinase family of site-specific recombinases. Members of this family of enzymes catalyze recombination between specific DNA sequences by cleaving and exchanging one pair of strands between the two substrate sites to form a 4-way Holliday junction (HJ) intermediate and then resolve the HJ intermediate to recombinant products by a second round of strand exchanges. Recently, hexapeptide inhibitors have been described that are capable of blocking the second strand exchange step in the tyrosine recombinase recombination pathway, leading to an accumulation of the HJ intermediate. These peptides are active in the lambda-integrase, Cre recombinase, and Flp recombinase systems and are potentially important tools for both in vitro mechanistic studies and as in vivo probes of cellular function. Here we present biochemical and crystallographic data that support a model where the peptide inhibitor binds in the center of the recombinase-bound DNA junction and interacts with solvent-exposed bases near the junction branch point. Peptide binding induces large conformational changes in the DNA strands of the HJ intermediate, which affect the active site geometries in the recombinase subunits.
Peptide trapping of the Holliday junction intermediate in Cre-loxP site-specific recombination.,Ghosh K, Lau CK, Guo F, Segall AM, Van Duyne GD J Biol Chem. 2005 Mar 4;280(9):8290-9. Epub 2004 Dec 8. PMID:15591069[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ghosh K, Lau CK, Guo F, Segall AM, Van Duyne GD. Peptide trapping of the Holliday junction intermediate in Cre-loxP site-specific recombination. J Biol Chem. 2005 Mar 4;280(9):8290-9. Epub 2004 Dec 8. PMID:15591069 doi:10.1074/jbc.M411668200
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