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1dhk

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[[Image:1dhk.gif|left|200px]]
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{{STRUCTURE_1dhk| PDB=1dhk | SCENE= }}
{{STRUCTURE_1dhk| PDB=1dhk | SCENE= }}
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'''STRUCTURE OF PORCINE PANCREATIC ALPHA-AMYLASE'''
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===STRUCTURE OF PORCINE PANCREATIC ALPHA-AMYLASE===
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==Overview==
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BACKGROUND: alpha-Amylases catalyze the hydrolysis of glycosidic linkages in starch and other related polysaccharides. The alpha-amylase inhibitor (alpha-Al) from the bean Phaseolus vulgaris belongs to a family of plant defence proteins and is a potent inhibitor of mammalian alpha-amylases. The structure of pig pancreatic alpha-amylase (PPA) in complex with both a carbohydrate inhibitor (acarbose) and a proteinaceous inhibitor (Tendamistat) is known, but the catalytic mechanism is poorly understood. RESULTS: The crystal structure of pig pancreatic alpha-amylase complexed with alpha-Al was refined to 1.85 A resolution. It reveals that in complex with PPA, the inhibitor has the typical dimer structure common to legume lectins. Two hairpin loops extending out from the jellyroll fold of a monomer interact directly with the active site region of the enzyme molecule, with the inhibitor molecule filling the whole substrate-docking region of the PPA. The inhibitor makes substrate-mimetic interactions with binding subsites of the enzyme and targets catalytic residues in the active site. Binding of inhibitor induces structural changes at the active site of the enzyme. CONCLUSIONS: The present analysis reveals that there are extensive interactions between the inhibitor and residues that are highly conserved in the active site of alpha-amylases; alpha-Al1 inactivates PPA through elaborate blockage of substrate-binding sites. It provides a basis to design peptide analogue inhibitors. alpha-Amylase inhibition is of interest from several points of view, for example the treatment of diabetes and for crop protection.
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(as it appears on PubMed at http://www.pubmed.gov), where 8994970 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8994970}}
==About this Structure==
==About this Structure==
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[[Category: Pancreatic alpha-amylase]]
[[Category: Pancreatic alpha-amylase]]
[[Category: Porcine]]
[[Category: Porcine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:51:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:04:32 2008''

Revision as of 20:04, 30 June 2008

Image:1dhk.png

Template:STRUCTURE 1dhk

STRUCTURE OF PORCINE PANCREATIC ALPHA-AMYLASE

Template:ABSTRACT PUBMED 8994970

About this Structure

1DHK is a Protein complex structure of sequences from Phaseolus vulgaris and Sus scrofa. Full crystallographic information is available from OCA.

Reference

Substrate mimicry in the active center of a mammalian alpha-amylase: structural analysis of an enzyme-inhibitor complex., Bompard-Gilles C, Rousseau P, Rouge P, Payan F, Structure. 1996 Dec 15;4(12):1441-52. PMID:8994970

Page seeded by OCA on Mon Jun 30 23:04:32 2008

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