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1diz

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[[Image:1diz.gif|left|200px]]
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{{STRUCTURE_1diz| PDB=1diz | SCENE= }}
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'''CRYSTAL STRUCTURE OF E. COLI 3-METHYLADENINE DNA GLYCOSYLASE (ALKA) COMPLEXED WITH DNA'''
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===CRYSTAL STRUCTURE OF E. COLI 3-METHYLADENINE DNA GLYCOSYLASE (ALKA) COMPLEXED WITH DNA===
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==Overview==
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The Escherichia coli AlkA protein is a base excision repair glycosylase that removes a variety of alkylated bases from DNA. The 2.5 A crystal structure of AlkA complexed to DNA shows a large distortion in the bound DNA. The enzyme flips a 1-azaribose abasic nucleotide out of DNA and induces a 66 degrees bend in the DNA with a marked widening of the minor groove. The position of the 1-azaribose in the enzyme active site suggests an S(N)1-type mechanism for the glycosylase reaction, in which the essential catalytic Asp238 provides direct assistance for base removal. Catalytic selectivity might result from the enhanced stacking of positively charged, alkylated bases against the aromatic side chain of Trp272 in conjunction with the relative ease of cleaving the weakened glycosylic bond of these modified nucleotides. The structure of the AlkA-DNA complex offers the first glimpse of a helix-hairpin-helix (HhH) glycosylase complexed to DNA. Modeling studies suggest that other HhH glycosylases can bind to DNA in a similar manner.
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(as it appears on PubMed at http://www.pubmed.gov), where 10675345 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10675345}}
==About this Structure==
==About this Structure==
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[[Category: Helix-hairpin-helix]]
[[Category: Helix-hairpin-helix]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:54:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:08:12 2008''

Revision as of 20:08, 30 June 2008


PDB ID 1diz

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1diz, resolution 2.50Å ()
Ligands:
Non-Standard Residues:
Activity: DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF E. COLI 3-METHYLADENINE DNA GLYCOSYLASE (ALKA) COMPLEXED WITH DNA

Template:ABSTRACT PUBMED 10675345

About this Structure

1DIZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

DNA bending and a flip-out mechanism for base excision by the helix-hairpin-helix DNA glycosylase, Escherichia coli AlkA., Hollis T, Ichikawa Y, Ellenberger T, EMBO J. 2000 Feb 15;19(4):758-66. PMID:10675345

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