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| - | [[Image:1djr.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1djr| PDB=1djr | SCENE= }} | | {{STRUCTURE_1djr| PDB=1djr | SCENE= }} |
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| - | '''HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH M-CARBOXYPHENYL-ALPHA-D-GALACTOSE'''
| + | ===HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH M-CARBOXYPHENYL-ALPHA-D-GALACTOSE=== |
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| - | ==Overview==
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| - | In the quest to develop drugs against traveller's diarrhoea and cholera, the structure of the B pentamer of heat-labile enterotoxin (LT) complexed with a new receptor-binding antagonist, m-carboxyphenyl-alpha-D-galactopyranoside, has been determined. The high resolution obtained for this structure allowed anisotropic refinement of the model. It was also now possible to confirm at a near-atomic resolution the structural similarity between the B subunits of LT and the closely related cholera toxin (CT), including the similarity in deviations of planarity of the same peptide unit in LT and CT. The structure of the LT complex clearly revealed different conformations for the m--carboxyphenyl moiety of the ligand in the five B subunits of LT, while the binding modes of the well defined galactopyranoside moieties were identical. In two binding sites the m-carboxyphenyl moiety displayed no significant electron density, demonstrating significant flexibility of this moiety. In a third binding site the m-carboxyphenyl moiety could be modelled unambiguously into the density. The two remaining binding sites were involved in crystal packing contacts and the density for the ligands in these two binding sites clearly revealed different binding modes, of which one conformation was identical to and one completely different from the conformation of m-carboxyphenyl-galactopyranoside in the third subunit. The multiple binding modes observed in the crystal may represent the ensemble of conformations of m-carboxyphenyl-alpha-D-galactopyranoside complexed to LT in solution.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10930826}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10930826 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10930826}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Cell recognition]] | | [[Category: Cell recognition]] |
| | [[Category: Six-stranded antiparallel beta-sheet]] | | [[Category: Six-stranded antiparallel beta-sheet]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:55:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:10:25 2008'' |
Revision as of 20:10, 30 June 2008
Template:STRUCTURE 1djr
HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH M-CARBOXYPHENYL-ALPHA-D-GALACTOSE
Template:ABSTRACT PUBMED 10930826
About this Structure
1DJR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of m-carboxyphenyl-alpha-D-galactopyranoside complexed to heat-labile enterotoxin at 1.3 A resolution: surprising variations in ligand-binding modes., Minke WE, Pickens J, Merritt EA, Fan E, Verlinde CL, Hol WG, Acta Crystallogr D Biol Crystallogr. 2000 Jul;56(Pt 7):795-804. PMID:10930826
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