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| | <StructureSection load='7khw' size='340' side='right'caption='[[7khw]], [[Resolution|resolution]] 3.40Å' scene=''> | | <StructureSection load='7khw' size='340' side='right'caption='[[7khw]], [[Resolution|resolution]] 3.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7khw]] is a 50 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KHW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7khw]] is a 50 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O127:H6_str._E2348/69 Escherichia coli O127:H6 str. E2348/69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KHW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7khw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khw OCA], [http://pdbe.org/7khw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7khw RCSB], [http://www.ebi.ac.uk/pdbsum/7khw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7khw ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7khw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7khw OCA], [https://pdbe.org/7khw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7khw RCSB], [https://www.ebi.ac.uk/pdbsum/7khw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7khw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/B7UM94_ECO27 B7UM94_ECO27] |
| - | Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC) utilize a macromolecular type III secretion system (T3SS) to inject effector proteins into eukaryotic cells. This apparatus spans the inner and outer bacterial membranes and includes a helical needle protruding into the extracellular space. Thus far observed only in EPEC and EHEC and not found in other pathogenic Gram-negative bacteria that have a T3SS is an additional helical filament made by the EspA protein that forms a long extension to the needle, mediating both attachment to eukaryotic cells and transport of effector proteins through the intestinal mucus layer. Here, we present the structure of the EspA filament from EPEC at 3.4 A resolution. The structure reveals that the EspA filament is a right-handed 1-start helical assembly with a conserved lumen architecture with respect to the needle to ensure the seamless transport of unfolded cargos en route to the target cell. This functional conservation is despite the fact that there is little apparent overall conservation at the level of sequence or structure with the needle. We also unveil the molecular details of the immunodominant EspA epitope that can now be exploited for the rational design of epitope display systems.
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| - | Cryoelectron-microscopy structure of the enteropathogenic Escherichia coli type III secretion system EspA filament.,Zheng W, Pena A, Ilangovan A, Clark JN, Frankel G, Egelman EH, Costa TRD Proc Natl Acad Sci U S A. 2021 Jan 12;118(2). pii: 2022826118. doi:, 10.1073/pnas.2022826118. PMID:33397726<ref>PMID:33397726</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 7khw" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eco27]] | + | [[Category: Escherichia coli O127:H6 str. E2348/69]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Costa, T R.D]] | + | [[Category: Costa TRD]] |
| - | [[Category: Egelman, E H]] | + | [[Category: Egelman EH]] |
| - | [[Category: Zheng, W]] | + | [[Category: Ilangovan A]] |
| - | [[Category: Espa filament]] | + | [[Category: Zheng W]] |
| - | [[Category: Helical reconstruction]]
| + | |
| - | [[Category: Protein fibril]]
| + | |
