|
|
| Line 3: |
Line 3: |
| | <StructureSection load='1ypy' size='340' side='right'caption='[[1ypy]], [[Resolution|resolution]] 1.51Å' scene=''> | | <StructureSection load='1ypy' size='340' side='right'caption='[[1ypy]], [[Resolution|resolution]] 1.51Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ypy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YPY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YPY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ypy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YPY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10245 Vaccinia virus])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ypy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ypy OCA], [http://pdbe.org/1ypy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ypy RCSB], [http://www.ebi.ac.uk/pdbsum/1ypy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ypy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ypy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ypy OCA], [https://pdbe.org/1ypy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ypy RCSB], [https://www.ebi.ac.uk/pdbsum/1ypy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ypy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/L1_VACCW L1_VACCW]] Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.<ref>PMID:8083978</ref> <ref>PMID:18596103</ref> <ref>PMID:19162289</ref> | + | [https://www.uniprot.org/uniprot/L1_VACCW L1_VACCW] Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.<ref>PMID:8083978</ref> <ref>PMID:18596103</ref> <ref>PMID:19162289</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 34: |
Line 34: |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Vaccinia virus]] | | [[Category: Vaccinia virus]] |
| - | [[Category: Allison, T J]] | + | [[Category: Allison TJ]] |
| - | [[Category: Fogg, C]] | + | [[Category: Fogg C]] |
| - | [[Category: Garboczi, D N]] | + | [[Category: Garboczi DN]] |
| - | [[Category: Garman, S C]] | + | [[Category: Garman SC]] |
| - | [[Category: Moss, B]] | + | [[Category: Moss B]] |
| - | [[Category: Su, H P]] | + | [[Category: Su HP]] |
| - | [[Category: L1]]
| + | |
| - | [[Category: Orthopox]]
| + | |
| - | [[Category: Smallpox]]
| + | |
| - | [[Category: Variola virus]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
L1_VACCW Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Although eradicated from nature more than two decades ago, the threat of smallpox has reemerged because of concerns over its use as a biological weapon. We present the structure of the poxvirus L1 protein, a molecule that is conserved throughout the poxvirus family and is nearly identical in vaccinia virus and in variola virus, which causes smallpox. L1 is a myristoylated envelope protein that is a potent target for neutralizing antibodies and an important component of current experimental vaccines. The L1 structure reveals a hydrophobic cavity located adjacent to its N terminus. The cavity would be capable of shielding the myristate moiety, which is essential for virion assembly. The structure of L1 is a step in the elucidation of molecular mechanisms common to all poxviruses that may stimulate the design of safer vaccines and new antipoxvirus drugs.
The 1.51-Angstrom structure of the poxvirus L1 protein, a target of potent neutralizing antibodies.,Su HP, Garman SC, Allison TJ, Fogg C, Moss B, Garboczi DN Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4240-5. Epub 2005 Mar 10. PMID:15761054[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ravanello MP, Hruby DE. Conditional lethal expression of the vaccinia virus L1R myristylated protein reveals a role in virion assembly. J Virol. 1994 Oct;68(10):6401-10. PMID:8083978
- ↑ Bisht H, Weisberg AS, Moss B. Vaccinia virus l1 protein is required for cell entry and membrane fusion. J Virol. 2008 Sep;82(17):8687-94. doi: 10.1128/JVI.00852-08. Epub 2008 Jul 2. PMID:18596103 doi:http://dx.doi.org/10.1128/JVI.00852-08
- ↑ Foo CH, Lou H, Whitbeck JC, Ponce-de-Leon M, Atanasiu D, Eisenberg RJ, Cohen GH. Vaccinia virus L1 binds to cell surfaces and blocks virus entry independently of glycosaminoglycans. Virology. 2009 Mar 15;385(2):368-82. doi: 10.1016/j.virol.2008.12.019. Epub 2009 , Jan 21. PMID:19162289 doi:http://dx.doi.org/10.1016/j.virol.2008.12.019
- ↑ Su HP, Garman SC, Allison TJ, Fogg C, Moss B, Garboczi DN. The 1.51-Angstrom structure of the poxvirus L1 protein, a target of potent neutralizing antibodies. Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4240-5. Epub 2005 Mar 10. PMID:15761054
|
