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| | <StructureSection load='1yy7' size='340' side='right'caption='[[1yy7]], [[Resolution|resolution]] 2.02Å' scene=''> | | <StructureSection load='1yy7' size='340' side='right'caption='[[1yy7]], [[Resolution|resolution]] 2.02Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1yy7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YY7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YY7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1yy7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YY7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1yy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yy7 OCA], [http://pdbe.org/1yy7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yy7 RCSB], [http://www.ebi.ac.uk/pdbsum/1yy7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yy7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yy7 OCA], [https://pdbe.org/1yy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yy7 RCSB], [https://www.ebi.ac.uk/pdbsum/1yy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yy7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A5P8YJZ1_YERPE A0A5P8YJZ1_YERPE] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 1yy7" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 1yy7" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Stringent starvation protein 3D structures|Stringent starvation protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Andrykovitch, M]] | + | [[Category: Yersinia pestis]] |
| - | [[Category: Gu, Y]] | + | [[Category: Andrykovitch M]] |
| - | [[Category: Hansen, A M]] | + | [[Category: Gu Y]] |
| - | [[Category: Ji, X]] | + | [[Category: Hansen A-M]] |
| - | [[Category: Jin, D J]] | + | [[Category: Ji X]] |
| - | [[Category: Li, M]] | + | [[Category: Jin DJ]] |
| - | [[Category: Waugh, D S]] | + | [[Category: Li M]] |
| - | [[Category: Gst fold]]
| + | [[Category: Waugh DS]] |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
A0A5P8YJZ1_YERPE
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Stringent starvation protein A (SspA) of Escherichia coli is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. We report the crystal structure of Yersinia pestis SspA, which is 83% identical to E. coli SspA in amino acid sequence and is functionally complementary in supporting the lytic growth of phage P1 and acid resistance of an E. coli sspA mutant. The structure reveals that SspA assumes the characteristic fold of glutathione S-transferase (GST). However, SspA lacks GST activity and does not bind glutathione. Three regions of SspA are flexible, the N and C termini and the alpha2-helix. The structure also reveals a conserved surface-exposed pocket composed of residues from a loop between helices alpha3 and alpha4. The functional roles of these structural features were investigated by assessing the ability of deletion and site-directed mutants to confer acid resistance of E. coli and to activate transcription from a phage P1 late promoter, thereby supporting the lytic growth of phage P1. The results indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. The size, shape, and property of the pocket suggest that it mediates protein-protein interactions. SspA orthologs from Y. pestis, Vibrio cholerae, and Pseudomonas aeruginosa are all functional in acid resistance of E. coli, whereas only Y. pestis SspA supports phage P1 growth.
Structural basis for the function of stringent starvation protein a as a transcription factor.,Hansen AM, Gu Y, Li M, Andrykovitch M, Waugh DS, Jin DJ, Ji X J Biol Chem. 2005 Apr 29;280(17):17380-91. Epub 2005 Feb 25. PMID:15735307[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hansen AM, Gu Y, Li M, Andrykovitch M, Waugh DS, Jin DJ, Ji X. Structural basis for the function of stringent starvation protein a as a transcription factor. J Biol Chem. 2005 Apr 29;280(17):17380-91. Epub 2005 Feb 25. PMID:15735307 doi:10.1074/jbc.M501444200
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