1dkc

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{{STRUCTURE_1dkc| PDB=1dkc | SCENE= }}
{{STRUCTURE_1dkc| PDB=1dkc | SCENE= }}
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'''SOLUTION STRUCTURE OF PAFP-S, AN ANTIFUNGAL PEPTIDE FROM THE SEEDS OF PHYTOLACCA AMERICANA'''
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===SOLUTION STRUCTURE OF PAFP-S, AN ANTIFUNGAL PEPTIDE FROM THE SEEDS OF PHYTOLACCA AMERICANA===
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==Overview==
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The three-dimensional solution structure of PAFP-S, an antifungal peptide extracted from the seeds of Phytolacca americana, was determined using 1H NMR spectroscopy. This cationic peptide contains 38 amino acid residues. Its structure was determined from 302 distance restraints and 36 dihedral restraints derived from NOEs and coupling constants. The peptide has six cysteines involved in three disulfide bonds. The previously unassigned parings have now been determined from NMR data. The solution structure of PAFP-S is presented as a set of 20 structures using ab initio dynamic simulated annealing, with an average RMS deviation of 1.68 A for the backbone heavy atoms and 2.19 A for all heavy atoms, respectively. For the well-defined triple-stranded beta-sheet involving residues 8-10, 23-27, and 32-36, the corresponding values were 0.39 and 1.25 A. The global fold involves a cystine-knotted three-stranded antiparallel beta-sheet (residues 8-10, 23-27, 32-36), a flexible loop (residues 14-19), and four beta-reverse turns (residues 4-8, 11-14, 19-22, 28-32). This structure features all the characteristics of the knottin fold. It is the first structural model of an antifungal peptide that adopts a knottin-type structure. PAFP-S has an extended hydrophobic surface comprised of residues Tyr23, Phe25, Ile27, Tyr32, and Val34. The side chains of these residues are well-defined in the NMR structure. Several hydrophilic and positively charged residues (Arg9, Arg38, and Lys36) surround the hydrophobic surface, giving PAFP-S an amphiphilic character which would be the main structural basis of its biological function.
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(as it appears on PubMed at http://www.pubmed.gov), where 11551192 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11551192}}
==About this Structure==
==About this Structure==
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1DKC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DKC OCA].
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1DKC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DKC OCA].
==Reference==
==Reference==
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[[Category: Wang, J F.]]
[[Category: Wang, J F.]]
[[Category: Three-strands beta sheet]]
[[Category: Three-strands beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:12:00 2008''

Revision as of 20:12, 30 June 2008

Image:1dkc.png

Template:STRUCTURE 1dkc

SOLUTION STRUCTURE OF PAFP-S, AN ANTIFUNGAL PEPTIDE FROM THE SEEDS OF PHYTOLACCA AMERICANA

Template:ABSTRACT PUBMED 11551192

About this Structure

1DKC is a Single protein structure of sequence from Phytolacca americana. Full experimental information is available from OCA.

Reference

Solution structure of PAFP-S: a new knottin-type antifungal peptide from the seeds of Phytolacca americana., Gao GH, Liu W, Dai JX, Wang JF, Hu Z, Zhang Y, Wang DC, Biochemistry. 2001 Sep 18;40(37):10973-8. PMID:11551192

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