6j2r
From Proteopedia
(Difference between revisions)
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==Crystal structure of Striga hermonthica HTL8 (ShHTL8)== | ==Crystal structure of Striga hermonthica HTL8 (ShHTL8)== | ||
| - | <StructureSection load='6j2r' size='340' side='right'caption='[[6j2r]]' scene=''> | + | <StructureSection load='6j2r' size='340' side='right'caption='[[6j2r]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J2R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j2r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Striga_hermonthica Striga hermonthica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J2R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j2r OCA], [https://pdbe.org/6j2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j2r RCSB], [https://www.ebi.ac.uk/pdbsum/6j2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j2r ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j2r OCA], [https://pdbe.org/6j2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j2r RCSB], [https://www.ebi.ac.uk/pdbsum/6j2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j2r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0M4APJ4_STRHE A0A0M4APJ4_STRHE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Striga is a parasitic weed that disperses easily, and its seeds can persist in the soil for many years, presenting long-term threats to food security. If SLs stimulate the seed germination of root parasitic weeds before planting, weeds will wither due to no host. Therefore, it is necessary to determine the mechanism of strigolactone (SL) signaling in Striga to reduce the impacts of this parasitic weed. Striga has eleven different kinds of HYPO-SENSITIVE to LIGHT (ShHTL) hydrolases. Different ShHTL hydrolases exhibit distinct responses to SLs, despite these ShHTLs exhibiting more than 60% sequence identity. Currently, structural information is available for only five ShHTL proteins, and more structural information is needed to design Striga germination stimulants or inhibitors. In this paper, we report the crystal structure of ShHTL8, which is determined at a resolution of 1.4 A. Scanning fluorimetry and HPLC assays indicate that L125, M147, M154 and I194 are important binding sites, and of which L125 may act as a key holder involved in the catalytic reaction. Additionally, the corresponding residue, Y124 of ShHTL1 and F135 of ShHTL2 also play a significant role in the substrate recognition. | ||
| + | |||
| + | Crystal structure and biochemical characterization of Striga hermonthica HYPO-SENSITIVE TO LIGHT 8 (ShHTL8) in strigolactone signaling pathway.,Zhang Y, Wang D, Shen Y, Xi Z Biochem Biophys Res Commun. 2020 Mar 19;523(4):1040-1045. doi:, 10.1016/j.bbrc.2020.01.056. Epub 2020 Jan 20. PMID:31973817<ref>PMID:31973817</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6j2r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Striga hermonthica]] | ||
[[Category: Xi Z]] | [[Category: Xi Z]] | ||
[[Category: Zhang YY]] | [[Category: Zhang YY]] | ||
Current revision
Crystal structure of Striga hermonthica HTL8 (ShHTL8)
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