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| | ==Solution NMR Structure of the Membrane Protein Stannin== | | ==Solution NMR Structure of the Membrane Protein Stannin== |
| - | <StructureSection load='1zza' size='340' side='right'caption='[[1zza]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='1zza' size='340' side='right'caption='[[1zza]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1zza]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZZA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1zza]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZZA FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SNN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zza OCA], [https://pdbe.org/1zza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zza RCSB], [https://www.ebi.ac.uk/pdbsum/1zza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zza ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zza OCA], [https://pdbe.org/1zza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zza RCSB], [https://www.ebi.ac.uk/pdbsum/1zza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zza ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/SNN_HUMAN SNN_HUMAN]] Plays a role in the toxic effects of organotins.
| + | [https://www.uniprot.org/uniprot/SNN_HUMAN SNN_HUMAN] Plays a role in the toxic effects of organotins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Buck-Koehntop, B A]] | + | [[Category: Buck-Koehntop BA]] |
| - | [[Category: Buffy, J J]] | + | [[Category: Buffy JJ]] |
| - | [[Category: Mascioni, A]] | + | [[Category: Mascioni A]] |
| - | [[Category: Veglia, G]] | + | [[Category: Veglia G]] |
| - | [[Category: Helix]]
| + | |
| - | [[Category: Membrane protein]]
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| Structural highlights
Function
SNN_HUMAN Plays a role in the toxic effects of organotins.
Publication Abstract from PubMed
Organotin compounds or alkyltins are ubiquitous environmental toxins that have been implicated in cellular death. Unlike other xenobiotic compounds, such as organomercurials and organoleads, alkyltins activate apoptotic cascades at low concentrations. Trimethyltin (TMT) chloride is amongst the most toxic organotin compounds, and is known to selectively inflict injury to specific regions of the brain. Stannin (SNN), an 88-residue mitochondrial membrane protein, has been identified as the specific marker for neuronal cell apoptosis induced by TMT intoxication. This high specificity of TMT makes SNN an ideal model system for understanding the mechanism of organotin neurotoxicity at a molecular level. Here, we report the three-dimensional structure and dynamics of SNN in detergent micelles, and its topological orientation in lipid bilayers as determined by solution and solid-state NMR spectroscopy. We found that SNN is a monotopic membrane protein composed of three domains: a single transmembrane helix (residues 10-33) that transverses the lipid bilayer at approximately a 20 degrees angle with respect to the membrane normal; a 28 residue unstructured linker, which includes a conserved CXC metal-binding motif and a putative 14-3-3zeta binding domain; and a distorted cytoplasmic helix (residues 61-79) that is partially absorbed into the plane of the lipid bilayer with a tilt angle of approximately 80 degrees from the membrane normal. The structure and architecture of SNN within the lipid environment provides insight about how this protein transmits toxic insults caused by TMT across the membrane.
Structure, dynamics, and membrane topology of stannin: a mediator of neuronal cell apoptosis induced by trimethyltin chloride.,Buck-Koehntop BA, Mascioni A, Buffy JJ, Veglia G J Mol Biol. 2005 Dec 2;354(3):652-65. Epub 2005 Sep 30. PMID:16246365[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Buck-Koehntop BA, Mascioni A, Buffy JJ, Veglia G. Structure, dynamics, and membrane topology of stannin: a mediator of neuronal cell apoptosis induced by trimethyltin chloride. J Mol Biol. 2005 Dec 2;354(3):652-65. Epub 2005 Sep 30. PMID:16246365 doi:S0022-2836(05)01104-6
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