Replication protein E1
From Proteopedia
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==Function== | ==Function== | ||
| - | '''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. | + | '''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. RPE1 is found also in bovine papillomavirus (BPV). |
== Disease == | == Disease == | ||
| - | + | RPE1 has been shown to mitigate host cell defenses leading to the development of cancer induces apoptosis of its host cells<ref>PMID:29495005</ref>. | |
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| + | ==Relevance== | ||
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| + | The detection of RPE1 mRNA and promoter methylation may serve as cancer prognostic marker. | ||
== Structural highlights == | == Structural highlights == | ||
| - | The complex between | + | The complex between BPV1 DBD and 21-bp DNA (PDB code [[1ksx]]) shows 2 DBD-DNA binding modules: a binding loop and a binding helix each interacting with a single strand. The protein-DNA interactions are hydrophilic to phosphate atoms and van der Waals hydrophobic ones<ref>PMID:11889054</ref>. |
</StructureSection> | </StructureSection> | ||
==3D structures of replication protein E1== | ==3D structures of replication protein E1== | ||
Revision as of 11:13, 27 January 2021
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3D structures of replication protein E1
Updated on 27-January-2021
Domains: helicase 301-605; DNA-binding (DBD) 286-365
1r9w - RPE1 DBD - HPV18
1tue - RPE1 428-631 (mutant) + RPE2 TAD
2v9p - RPE1 helicase domain - BPV1
5a9k - RPE1 helicase domain - Cryo EM
2gxa - RPE1 helicase domain + DNA + ADP
1f08 - RPE1 DBD
1ksx, 1ksy - RPE1 DBD + DNA
References
- ↑ Wilson VG, West M, Woytek K, Rangasamy D. Papillomavirus E1 proteins: form, function, and features. Virus Genes. 2002 Jun;24(3):275-90. doi: 10.1023/a:1015336817836. PMID:12086149 doi:http://dx.doi.org/10.1023/a:1015336817836
- ↑ Baedyananda F, Chaiwongkot A, Bhattarakosol P. Elevated HPV16 E1 Expression Is Associated with Cervical Cancer Progression. Intervirology. 2017;60(5):171-180. doi: 10.1159/000487048. Epub 2018 Mar 1. PMID:29495005 doi:http://dx.doi.org/10.1159/000487048
- ↑ Enemark EJ, Stenlund A, Joshua-Tor L. Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex. EMBO J. 2002 Mar 15;21(6):1487-96. PMID:11889054 doi:http://dx.doi.org/10.1093/emboj/21.6.1487
