2nu5
From Proteopedia
(New page: 200px<br /> <applet load="2nu5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nu5, resolution 1.564Å" /> '''Crystal structure ...) |
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| - | [[Image:2nu5. | + | [[Image:2nu5.jpg|left|200px]]<br /><applet load="2nu5" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2nu5" size=" | + | |
caption="2nu5, resolution 1.564Å" /> | caption="2nu5, resolution 1.564Å" /> | ||
'''Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine'''<br /> | '''Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NU5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia_sp._q66d336 Griffithsia sp. q66d336] with NAG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2NU5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia_sp._q66d336 Griffithsia sp. q66d336] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NU5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:16:45 2008'' |
Revision as of 12:16, 23 January 2008
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Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT), with glucose and N-acetylglucosamine were solved and refined at high, resolution. In both complexes, all six monosaccharide-binding sites of, GRFT were occupied and the mode of binding was similar to that of mannose., In our previous attempts to obtain a complex with N-acetylglucosamine by, soaking, only a single site was occupied; thus, cocrystallization was, clearly superior despite lower concentration of the ligand. Isothermal, titration calorimetric experiments with N-acetylglucosamine, glucose, and, mannose provided enthalpic evidence of distinct binding differences, between the three monosaccharides. A comparison of the mode of binding of, different monosaccharides is discussed in the context of the antiviral, activity of GRFT, based on specific binding to high-mannose-containing, complex carbohydrates found on viral envelopes.
About this Structure
2NU5 is a Single protein structure of sequence from Griffithsia sp. q66d336 with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
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