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6yu8
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==RNA Methyltransferase of Sudan Ebola Virus== |
| - | <StructureSection load='6yu8' size='340' side='right'caption='[[6yu8]]' scene=''> | + | <StructureSection load='6yu8' size='340' side='right'caption='[[6yu8]], [[Resolution|resolution]] 1.84Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yu8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Sudan_virus_-_Boniface,_Sudan,1976 Sudan virus - Boniface, Sudan,1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YU8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu8 OCA], [https://pdbe.org/6yu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yu8 RCSB], [https://www.ebi.ac.uk/pdbsum/6yu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu8 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.841Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu8 OCA], [https://pdbe.org/6yu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yu8 RCSB], [https://www.ebi.ac.uk/pdbsum/6yu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/L_EBOSU L_EBOSU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Ebola virus is a deadly human pathogen responsible for several outbreaks in Africa. Its genome encodes the 'large' L protein, an essential enzyme that has polymerase, capping and methyltransferase activities. The methyltransferase activity leads to RNA co-transcriptional modifications at the N7 position of the cap structure and at the 2'-O position of the first transcribed nucleotide. Unlike other Mononegavirales viruses, the Ebola virus methyltransferase also catalyses 2'-O-methylation of adenosines located within the RNA sequences. Herein, we report the crystal structure at 1.8 A resolution of the Ebola virus methyltransferase domain bound to a fragment of a camelid single-chain antibody. We identified structural determinants and key amino acids specifically involved in the internal adenosine-2'-O-methylation from cap-related methylations. These results provide the first high resolution structure of an ebolavirus L protein domain, and the framework to investigate the effects of epitranscriptomic modifications and to design possible antiviral drugs against the Filoviridae family. | ||
| + | |||
| + | First insights into the structural features of Ebola virus methyltransferase activities.,Valle C, Martin B, Ferron F, Roig-Zamboni V, Desmyter A, Debart F, Vasseur JJ, Canard B, Coutard B, Decroly E Nucleic Acids Res. 2021 Feb 22;49(3):1737-1748. doi: 10.1093/nar/gkaa1276. PMID:33503246<ref>PMID:33503246</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yu8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Lama glama]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Sudan virus - Boniface, Sudan,1976]] |
| + | [[Category: Canard B]] | ||
| + | [[Category: Decroly E]] | ||
| + | [[Category: Ferron F]] | ||
| + | [[Category: Valle C]] | ||
| + | [[Category: Zamboni V]] | ||
Current revision
RNA Methyltransferase of Sudan Ebola Virus
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