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6zfd

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==14-3-3 zeta bound to the phosphorylated 18E6 C-terminus==
==14-3-3 zeta bound to the phosphorylated 18E6 C-terminus==
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<StructureSection load='6zfd' size='340' side='right'caption='[[6zfd]]' scene=''>
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<StructureSection load='6zfd' size='340' side='right'caption='[[6zfd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZFD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6zfd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZFD FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zfd OCA], [https://pdbe.org/6zfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zfd RCSB], [https://www.ebi.ac.uk/pdbsum/6zfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zfd ProSAT]</span></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">E6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zfd OCA], [https://pdbe.org/6zfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zfd RCSB], [https://www.ebi.ac.uk/pdbsum/6zfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zfd ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/1433Z_HUMAN 1433Z_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.<ref>PMID:9360956</ref> <ref>PMID:14578935</ref> <ref>PMID:15071501</ref> <ref>PMID:15644438</ref> <ref>PMID:16376338</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The seven 14-3-3 isoforms are highly abundant human proteins encoded by similar yet distinct genes. 14-3-3 proteins recognize phosphorylated motifs within numerous human and viral proteins. Here, we analyze by X-ray crystallography, fluorescence polarization, mutagenesis and fusicoccin-mediated modulation the structural basis and druggability of 14-3-3 binding to four E6 oncoproteins of tumorigenic human papillomaviruses. 14-3-3 isoforms bind variant and mutated phospho-motifs of E6 and unrelated protein RSK1 with different affinities, albeit following an ordered affinity ranking with conserved relative KD ratios. Remarkably, 14-3-3 isoforms obey the same hierarchy when binding to most of their established targets, as supported by literature and a recent human complexome map. This knowledge allows predicting proportions of 14-3-3 isoforms engaged with phosphoproteins in various tissues. Notwithstanding their individual functions, cellular concentrations of 14-3-3 may be collectively adjusted to buffer the strongest phosphorylation outbursts, explaining their expression variations in different tissues and tumors.
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Hierarchized phosphotarget binding by the seven human 14-3-3 isoforms.,Gogl G, Tugaeva KV, Eberling P, Kostmann C, Trave G, Sluchanko NN Nat Commun. 2021 Mar 15;12(1):1677. doi: 10.1038/s41467-021-21908-8. PMID:33723253<ref>PMID:33723253</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6zfd" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Gogl G]]
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[[Category: Gogl, G]]
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[[Category: Sluchanko NN]]
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[[Category: Sluchanko, N N]]
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[[Category: Trave G]]
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[[Category: Trave, G]]
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[[Category: Tugaeva KV]]
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[[Category: Tugaeva, K V]]
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[[Category: 14-3-3]]
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[[Category: E6 oncoprotein]]
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[[Category: Hpv]]
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[[Category: Protein binding]]

Revision as of 03:02, 21 April 2022

14-3-3 zeta bound to the phosphorylated 18E6 C-terminus

PDB ID 6zfd

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