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Protein Hfq

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== Function ==
== Function ==
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'''Protein Hfq''' (Hfq) ('''H'''ost '''F'''actor for '''Q'''β) or '''RNA-binding protein Hfq''' is stimulating base-pairing between sRNA and target mRNA by binding both RNAs via three RNA-binding surfaces. Hfq is found in enteric bacteria<ref>PMID:30487269</ref>. SAP binds in a calcium-dependent fashion to a variety of ligands.
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'''Protein Hfq''' (Hfq) ('''H'''ost '''F'''actor for '''Q'''β) or '''RNA-binding protein Hfq''' is stimulating base-pairing between sRNA and target mRNA by binding both RNAs via three RNA-binding surfaces. Hfq is found in enteric bacteria<ref>PMID:30487269</ref>. Hfq binds RNA via poly(A-R-N) triplets where A is adenosine, R is purine nucleotide and N is any nucleotide<ref>PMID:19889981</ref>.
== Relevance ==
== Relevance ==
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== Structural highlights ==
== Structural highlights ==
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<scene name='87/875651/Cv/5'>The 3D structure of a complex of SAP with the small molecule ligand AMP</scene> shows the nucleotide phosphate group bridging two Ca+2 ions and forming hydrogen bonds to Asn, Gln and Try residues of SAP <ref>PMID:9217261</ref>.
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The 3D structure of the complex between Hfq and poly(A) RNA and poly(U) RNA shows the poly(A) binding at the distal tase of Hfq and poly(U) binding at its proximal face. In the poly(A) A-R-N binding site the A site shows hydrogen bonding to Gln and Lys. The R site site shows stacking interactions as well as hydrogen bonding. The N site does not show any interaction. The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions<ref>PMID:23605038</ref>.
</StructureSection>
</StructureSection>

Revision as of 08:37, 22 February 2021

Structure of E. coli trimeric Hfq complex with poly(A) RNA (yellow, cyan) and poly(U) RNA (magenta, green) (PDB code 4ht9)

Drag the structure with the mouse to rotate

3D Structures of protein Hfq

Updated on 22-February-2021


References

  1. ↑ Morita T, Aiba H. Mechanism and physiological significance of autoregulation of the Escherichia coli hfq gene. RNA. 2019 Feb;25(2):264-276. doi: 10.1261/rna.068106.118. Epub 2018 Nov 28. PMID:30487269 doi:http://dx.doi.org/10.1261/rna.068106.118
  2. ↑ Link TM, Valentin-Hansen P, Brennan RG. Structure of Escherichia coli Hfq bound to polyriboadenylate RNA. Proc Natl Acad Sci U S A. 2009 Nov 4. PMID:19889981
  3. ↑ Lybecker MC, Abel CA, Feig AL, Samuels DS. Identification and function of the RNA chaperone Hfq in the Lyme disease spirochete Borrelia burgdorferi. Mol Microbiol. 2010 Nov;78(3):622-35. doi: 10.1111/j.1365-2958.2010.07374.x. Epub, 2010 Sep 27. PMID:20815822 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07374.x
  4. ↑ Wang W, Wang L, Wu J, Gong Q, Shi Y. Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA. Nucleic Acids Res. 2013 Jun 1;41(11):5938-48. doi: 10.1093/nar/gkt276. Epub 2013 , Apr 19. PMID:23605038 doi:10.1093/nar/gkt276

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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