7brs
From Proteopedia
(Difference between revisions)
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==E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA02== | ==E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA02== | ||
| - | <StructureSection load='7brs' size='340' side='right'caption='[[7brs]]' scene=''> | + | <StructureSection load='7brs' size='340' side='right'caption='[[7brs]], [[Resolution|resolution]] 2.67Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BRS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7brs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BRS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7brs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7brs OCA], [https://pdbe.org/7brs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7brs RCSB], [https://www.ebi.ac.uk/pdbsum/7brs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7brs ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=F4X:8-[2-[(E)-2-[4-[(2S,3R,4S,5R,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxyphenyl]ethenyl]-3,3-dimethyl-indol-1-ium-1-yl]octanoic+acid'>F4X</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7brs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7brs OCA], [https://pdbe.org/7brs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7brs RCSB], [https://www.ebi.ac.uk/pdbsum/7brs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7brs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The tracking of cellular senescence usually depends on the detection of senescence-associated beta-galactosidase (SA-beta-gal). Previous probes for SA-beta-gal with this purpose only cover a single dimension: the accumulation of this enzyme in lysosomes. However, this is insufficient to determine the destiny of senescence because endogenous beta-gal enriched in lysosomes is not only related to senescence, but also to some other physiological processes. To address this issue, we introduce our fluorescent probes including a second dimension: lysosomal pH, since de-acidification is a unique feature of the lysosomes in senescent cells. With this novel design, our probes achieved excellent discrimination of SA-beta-gal from cancer-associated beta-gal, which enables them to track cellular senescence as well as tissue aging more precisely. Our crystal structures of a model enzyme E. coli beta-gal mutant (E537Q) complexed with each probe further revealed the structural basis for probe recognition. | ||
| + | |||
| + | Two-Dimensional Design Strategy to Construct Smart Fluorescent Probes for the Precise Tracking of Senescence.,Guo Y, Gao Y, Hu Y, Liu Q, Li X, Li X, Kim CY, James TD, Li J, Chen X Angew Chem Int Ed Engl. 2021 Feb 24. doi: 10.1002/anie.202101278. PMID:33624914<ref>PMID:33624914</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7brs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Beta-galactosidase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chen X]] | + | [[Category: Chen, X]] |
| - | [[Category: Gao Y]] | + | [[Category: Gao, Y]] |
| - | [[Category: Guo Y]] | + | [[Category: Guo, Y]] |
| - | [[Category: Hu | + | [[Category: Hu, Y L]] |
| - | [[Category: Yuan R]] | + | [[Category: Yuan, R]] |
| + | [[Category: Beta_galactosidase]] | ||
| + | [[Category: Fluorescent probe]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 17:56, 10 March 2021
E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA02
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