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6wxw

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Current revision (14:47, 6 March 2024) (edit) (undo)
 
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<StructureSection load='6wxw' size='340' side='right'caption='[[6wxw]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
<StructureSection load='6wxw' size='340' side='right'caption='[[6wxw]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6wxw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tres6 Tres6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WXW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6wxw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_succinifaciens_DSM_2489 Treponema succinifaciens DSM 2489]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WXW FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tresu_2185 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=869209 TRES6])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wxw OCA], [https://pdbe.org/6wxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wxw RCSB], [https://www.ebi.ac.uk/pdbsum/6wxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wxw ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wxw OCA], [https://pdbe.org/6wxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wxw RCSB], [https://www.ebi.ac.uk/pdbsum/6wxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wxw ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/F2NWD3_TRES6 F2NWD3_TRES6]
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In the type III CRISPR-Cas immune response of prokaryotes, infection triggers the production of cyclic oligoadenylates that bind and activate proteins that contain a CARF domain(1,2). Many type III loci are associated with proteins in which the CRISPR-associated Rossman fold (CARF) domain is fused to a restriction endonuclease-like domain(3,4). However, with the exception of the well-characterized Csm6 and Csx1 ribonucleases(5,6), whether and how these inducible effectors provide defence is not known. Here we investigated a type III CRISPR accessory protein, which we name cyclic-oligoadenylate-activated single-stranded ribonuclease and single-stranded deoxyribonuclease 1 (Card1). Card1 forms a symmetrical dimer that has a large central cavity between its CRISPR-associated Rossmann fold and restriction endonuclease domains that binds cyclic tetra-adenylate. The binding of ligand results in a conformational change comprising the rotation of individual monomers relative to each other to form a more compact dimeric scaffold, in which a manganese cation coordinates the catalytic residues and activates the cleavage of single-stranded-but not double-stranded-nucleic acids (both DNA and RNA). In vivo, activation of Card1 induces dormancy of the infected hosts to provide immunity against phage infection and plasmids. Our results highlight the diversity of strategies used in CRISPR systems to provide immunity.
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The Card1 nuclease provides defence during type III CRISPR immunity.,Rostol JT, Xie W, Kuryavyi V, Maguin P, Kao K, Froom R, Patel DJ, Marraffini LA Nature. 2021 Feb;590(7847):624-629. doi: 10.1038/s41586-021-03206-x. Epub 2021, Jan 18. PMID:33461211<ref>PMID:33461211</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6wxw" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Tres6]]
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[[Category: Treponema succinifaciens DSM 2489]]
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[[Category: Marraffini, L]]
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[[Category: Marraffini L]]
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[[Category: Patel, D J]]
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[[Category: Patel DJ]]
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[[Category: Rostol, J]]
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[[Category: Rostol J]]
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[[Category: Xie, W]]
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[[Category: Xie W]]
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[[Category: Card1]]
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[[Category: Cyclic oligoadenylate]]
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[[Category: Dna binding protein]]
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[[Category: Dnase]]
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Current revision

crystal structure of apo Card1

PDB ID 6wxw

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