1dg2

From Proteopedia

(Difference between revisions)

Revision as of 11:44, 22 November 2023

SOLUTION CONFORMATION OF A-CONOTOXIN AUIB

Structural highlights

1dg2 is a 1 chain structure with sequence from Conus aulicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CA1B_CONAL Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.^[1]

Publication Abstract from PubMed

The neuronal nicotinic acetylcholine receptors constitute a highly diverse group, with subtypes consisting of pentameric combinations of alpha and beta subunits. alpha-Conotoxins are a homologous series of small peptides that antagonize these receptors. We present the three-dimensional solution structure of alpha-conotoxin AuIB, the first 15-residue alpha-conotoxin known to selectively block the alpha(3)beta(4) nicotinic acetylcholine receptor subtype. The pairwise backbone and heavy-atom root mean square deviation for an ensemble of 20 structures are 0.269 and 0.720 A, respectively. The overall fold of alpha-conotoxin AuIB closely resembles that of the alpha4/7 subfamily alpha-conotoxins. However, the absence of Tyr(15), normally present in other alpha4/7 members, results in tight bending of the backbone at the C terminus and effectively renders Asp(14) to assume the spatial location of Tyr(15) present in other neuronal alpha4/7 alpha-conotoxins. Structural comparison of alpha-conotoxin AuIB with the alpha(3)beta(2) subtype-specific alpha-conotoxin MII shows different electrostatic surface charge distributions, which may be important in differential receptor subtype recognition.

Nuclear magnetic resonance solution conformation of alpha-conotoxin AuIB, an alpha(3)beta(4) subtype-selective neuronal nicotinic acetylcholine receptor antagonist.,Cho JH, Mok KH, Olivera BM, McIntosh JM, Park KH, Han KH J Biol Chem. 2000 Mar 24;275(12):8680-5. PMID:10722709^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luo S, Kulak JM, Cartier GE, Jacobsen RB, Yoshikami D, Olivera BM, McIntosh JM. alpha-conotoxin AuIB selectively blocks alpha3 beta4 nicotinic acetylcholine receptors and nicotine-evoked norepinephrine release. J Neurosci. 1998 Nov 1;18(21):8571-9. PMID:9786965
↑ Cho JH, Mok KH, Olivera BM, McIntosh JM, Park KH, Han KH. Nuclear magnetic resonance solution conformation of alpha-conotoxin AuIB, an alpha(3)beta(4) subtype-selective neuronal nicotinic acetylcholine receptor antagonist. J Biol Chem. 2000 Mar 24;275(12):8680-5. PMID:10722709

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1dg2"

@@ Line 1: / Line 1: @@
 ==SOLUTION CONFORMATION OF A-CONOTOXIN AUIB==
-<StructureSection load='1dg2' size='340' side='right'caption='[[1dg2]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1dg2' size='340' side='right'caption='[[1dg2]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1dg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DG2 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dg2 OCA], [https://pdbe.org/1dg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1dg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dg2 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CA1B_CONAL CA1B_CONAL]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.<ref>PMID:9786965</ref>
+[https://www.uniprot.org/uniprot/CA1B_CONAL CA1B_CONAL] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.<ref>PMID:9786965</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 [[Category: Conus aulicus]]
 [[Category: Large Structures]]
-[[Category: Cho, J H]]
+[[Category: Cho J-H]]
-[[Category: Han, K H]]
+[[Category: Han K-H]]
-[[Category: McIntosh, J M]]
+[[Category: McIntosh JM]]
-[[Category: Mok, K H]]
+[[Category: Mok KH]]
-[[Category: Olivera, B M]]
+[[Category: Olivera BM]]
-[[Category: Park, K H]]
+[[Category: Park K-H]]
-[[Category: A-helix]]
-[[Category: Toxin]]
-[[Category: Two disulfide bonds and c-term amidation]]

1dg2

From Proteopedia

Revision as of 11:44, 22 November 2023

SOLUTION CONFORMATION OF A-CONOTOXIN AUIB

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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