2gqe

From Proteopedia

(Difference between revisions)

Current revision

Molecular characterization of the Ran binding zinc finger domain

Structural highlights

2gqe is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NU153_HUMAN Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The nuclear pore complex is the gateway for selective traffic between the nucleus and cytoplasm. To learn how building blocks of the pore can create specific docking sites for transport receptors and regulatory factors, we have studied a zinc finger module present in multiple copies within the nuclear pores of higher eukaryotes. All four zinc fingers of human Nup153 were found to bind the small GTPase Ran with dissociation constants ranging between 5 and 40 mum. In addition a fragment of Nup153 encompassing the four tandem zinc fingers was found to bind Ran with similar affinity. NMR structural studies revealed that a representative Nup153 zinc finger adopts the same zinc ribbon structure as the previously characterized Npl4 NZF module. Ran binding was mediated by a three-amino acid motif (Leu(13)/Val(14)/Asn(25)) located within the two zinc coordination loops. Nup153 ZnFs bound GDP and GTP forms of Ran with similar affinities, indicating that this interaction is not influenced by a nucleotide-dependent conformational switch. Taken together, these studies elucidate the Ran-binding interface on Nup153 and, more broadly, provide insight into the versatility of this zinc finger binding module.

Molecular characterization of the Ran-binding zinc finger domain of Nup153.,Higa MM, Alam SL, Sundquist WI, Ullman KS J Biol Chem. 2007 Jun 8;282(23):17090-100. Epub 2007 Apr 10. PMID:17426026^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Higa MM, Alam SL, Sundquist WI, Ullman KS. Molecular characterization of the Ran-binding zinc finger domain of Nup153. J Biol Chem. 2007 Jun 8;282(23):17090-100. Epub 2007 Apr 10. PMID:17426026 doi:10.1074/jbc.M702715200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gqe"

@@ Line 1: / Line 1: @@
 ==Molecular characterization of the Ran binding zinc finger domain==
-<StructureSection load='2gqe' size='340' side='right'caption='[[2gqe]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2gqe' size='340' side='right'caption='[[2gqe]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gqe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GQE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gqe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GQE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1nj3|1nj3]], [[1q5w|1q5w]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NUP153 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gqe OCA], [https://pdbe.org/2gqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gqe RCSB], [https://www.ebi.ac.uk/pdbsum/2gqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gqe ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/NU153_HUMAN NU153_HUMAN]] Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
+[https://www.uniprot.org/uniprot/NU153_HUMAN NU153_HUMAN] Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 37: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Alam, S L]]
+[[Category: Alam SL]]
-[[Category: Higa, M M]]
+[[Category: Higa MM]]
-[[Category: Sundquist, W I]]
+[[Category: Sundquist WI]]
-[[Category: Ullman, K S]]
+[[Category: Ullman KS]]
-[[Category: Nuclear pore]]
-[[Category: Nucleoporin]]
-[[Category: Nup153]]
-[[Category: Nup358]]
-[[Category: Pore]]
-[[Category: Ran]]
-[[Category: Ranbp2]]
-[[Category: Transport]]
-[[Category: Transport protein]]
-[[Category: Zinc finger]]
-[[Category: Zinc knuckle]]

2gqe

From Proteopedia

Current revision

Molecular characterization of the Ran binding zinc finger domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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