6wpc
From Proteopedia
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| - | ==Crystal structure of Bacillus thuringiensis | + | ==Crystal structure of Bacillus thuringiensis Cry1A.2 tryptic core variant== |
| - | <StructureSection load='6wpc' size='340' side='right'caption='[[6wpc]]' scene=''> | + | <StructureSection load='6wpc' size='340' side='right'caption='[[6wpc]], [[Resolution|resolution]] 2.99Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wpc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPC FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpc OCA], [https://pdbe.org/6wpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpc RCSB], [https://www.ebi.ac.uk/pdbsum/6wpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpc ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpc OCA], [https://pdbe.org/6wpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpc RCSB], [https://www.ebi.ac.uk/pdbsum/6wpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Two new chimeric Bacillus thuringiensis (Bt) proteins, Cry1A.2 and Cry1B.2, were constructed using specific domains, which provide insecticidal activity against key lepidopteran soybean pests while minimizing receptor overlaps between themselves, current, and soon to be commercialized plant incorporated protectants (PIP's) in soybean. Results from insect diet bioassays demonstrate that the recombinant Cry1A.2 and Cry1B.2 are toxic to soybean looper (SBL) Chrysodeixis includens Walker, velvetbean caterpillar (VBC) Anticarsia gemmatalis Hubner, southern armyworm (SAW) Spodoptera eridania, and black armyworm (BLAW) Spodoptera cosmioides with LC50 values < 3,448 ng/cm2. Cry1B.2 is of moderate activity with significant mortality and stunting at > 3,448 ng/cm2, while Cry1A.2 lacks toxicity against old-world bollworm (OWB) Helicoverpa armigera. Results from disabled insecticidal protein (DIP) bioassays suggest that receptor utilization of Cry1A.2 and Cry1B.2 proteins are distinct from each other and from current, and yet to be commercially available, Bt proteins in soy such as Cry1Ac, Cry1A.105, Cry1F.842, Cry2Ab2 and Vip3A. However, as Cry1A.2 contains a domain common to at least one commercial soybean Bt protein, resistance to this common domain in a current commercial soybean Bt protein could possibly confer at least partial cross resistance to Cry1A2. Therefore, Cry1A.2 and Cry1B.2 should provide two new tools for controlling many of the major soybean insect pests described above. | ||
| + | |||
| + | Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.,Chen D, Moar WJ, Jerga A, Gowda A, Milligan JS, Bretsynder EC, Rydel TJ, Baum JA, Semeao A, Fu X, Guzov V, Gabbert K, Head GP, Haas JA PLoS One. 2021 Jun 17;16(6):e0249150. doi: 10.1371/journal.pone.0249150., eCollection 2021. PMID:34138865<ref>PMID:34138865</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wpc" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Delta-endotoxin|Delta-endotoxin]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus cereus var. thuringiensis smith et al. 1952]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Evdokimov A]] | + | [[Category: Evdokimov, A]] |
| - | [[Category: Rydel | + | [[Category: Rydel, T J]] |
| + | [[Category: 3-domain cry]] | ||
| + | [[Category: B t]] | ||
| + | [[Category: Bacillus thuringiensis]] | ||
| + | [[Category: Toxin]] | ||
Revision as of 08:55, 29 September 2021
Crystal structure of Bacillus thuringiensis Cry1A.2 tryptic core variant
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