7ci3

From Proteopedia

(Difference between revisions)

Revision as of 16:08, 29 November 2023

The crystal structure of the SARS-CoV-2 ORF7a ectodomain

Structural highlights

7ci3 is a 1 chain structure with sequence from Severe acute respiratory syndrome coronavirus 2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NS7A_SARS2 Non-structural protein which is dispensable for virus replication in cell culture.[UniProtKB:P59635]

Publication Abstract from PubMed

Dysregulated immune cell responses have been linked to the severity of coronavirus disease 2019 (COVID-19), but the specific viral factors of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were currently unknown. Herein, we reveal that the Immunoglobulin-like fold ectodomain of the viral protein SARS-CoV-2 ORF7a interacts with high efficiency to CD14(+) monocytes in human peripheral blood, compared to pathogenic protein SARS-CoV ORF7a. The crystal structure of SARS-CoV-2 ORF7a at 2.2 A resolution reveals three remarkable changes on the amphipathic side of the four-stranded beta-sheet, implying a potential functional interface of the viral protein. Importantly, SARS-CoV-2 ORF7a coincubation with CD14(+) monocytes ex vivo triggered a decrease in HLA-DR/DP/DQ expression levels and upregulated significant production of proinflammatory cytokines, including IL-6, IL-1beta, IL-8, and TNF-alpha. Our work demonstrates that SARS-CoV-2 ORF7a is an immunomodulating factor for immune cell binding and triggers dramatic inflammatory responses, providing promising therapeutic drug targets for pandemic COVID-19.

Structural insight reveals SARS-CoV-2 ORF7a as an immunomodulating factor for human CD14(+) monocytes.,Zhou Z, Huang C, Zhou Z, Huang Z, Su L, Kang S, Chen X, Chen Q, He S, Rong X, Xiao F, Chen J, Chen S iScience. 2021 Mar 19;24(3):102187. doi: 10.1016/j.isci.2021.102187. Epub 2021, Feb 12. PMID:33615195^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou Z, Huang C, Zhou Z, Huang Z, Su L, Kang S, Chen X, Chen Q, He S, Rong X, Xiao F, Chen J, Chen S. Structural insight reveals SARS-CoV-2 ORF7a as an immunomodulating factor for human CD14(+) monocytes. iScience. 2021 Mar 19;24(3):102187. doi: 10.1016/j.isci.2021.102187. Epub 2021, Feb 12. PMID:33615195 doi:http://dx.doi.org/10.1016/j.isci.2021.102187

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7ci3"

Categories: Large Structures | Severe acute respiratory syndrome coronavirus 2 | Chen S | Zhou Z

@@ Line 3: / Line 3: @@
 <StructureSection load='7ci3' size='340' side='right'caption='[[7ci3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7ci3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CI3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7ci3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CI3 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF7a, NS7a, orf7a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ci3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ci3 OCA], [https://pdbe.org/7ci3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ci3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ci3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ci3 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/NS7A_SARS2 NS7A_SARS2] Non-structural protein which is dispensable for virus replication in cell culture.[UniProtKB:P59635]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 </div>
 <div class="pdbe-citations 7ci3" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: 2019-ncov]]
 [[Category: Large Structures]]
-[[Category: Chen, S]]
+[[Category: Severe acute respiratory syndrome coronavirus 2]]
-[[Category: Zhou, Z]]
+[[Category: Chen S]]
-[[Category: Accessory protein]]
+[[Category: Zhou Z]]
-[[Category: Er membrane]]
-[[Category: Ig-like fold]]
-[[Category: Type-i transmembrane protein]]
-[[Category: Viral protein]]

7ci3

From Proteopedia

Revision as of 16:08, 29 November 2023

The crystal structure of the SARS-CoV-2 ORF7a ectodomain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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