6e1o

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Current revision (14:38, 13 March 2024) (edit) (undo)
 
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<SX load='6e1o' size='340' side='right' viewer='molstar' caption='[[6e1o]], [[Resolution|resolution]] 3.59&Aring;' scene=''>
<SX load='6e1o' size='340' side='right' viewer='molstar' caption='[[6e1o]], [[Resolution|resolution]] 3.59&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6e1o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E1O FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6e1o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E1O FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.59&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6dz7|6dz7]], [[6e0h|6e0h]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFUA_4G02970 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1o OCA], [https://pdbe.org/6e1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e1o RCSB], [https://www.ebi.ac.uk/pdbsum/6e1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1o ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1o OCA], [https://pdbe.org/6e1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e1o RCSB], [https://www.ebi.ac.uk/pdbsum/6e1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1o ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q4WA18_ASPFU Q4WA18_ASPFU]
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The lipid distribution of plasma membranes of eukaryotic cells is asymmetric and phospholipid scramblases disrupt this asymmetry by mediating the rapid, nonselective transport of lipids down their concentration gradients. As a result, phosphatidylserine is exposed to the outer leaflet of membrane, an important step in extracellular signaling networks controlling processes such as apoptosis, blood coagulation, membrane fusion and repair. Several TMEM16 family members have been identified as Ca(2+)-activated scramblases, but the mechanisms underlying their Ca(2+)-dependent gating and their effects on the surrounding lipid bilayer remain poorly understood. Here, we describe three high-resolution cryo-electron microscopy structures of a fungal scramblase from Aspergillus fumigatus, afTMEM16, reconstituted in lipid nanodiscs. These structures reveal that Ca(2+)-dependent activation of the scramblase entails global rearrangement of the transmembrane and cytosolic domains. These structures, together with functional experiments, suggest that activation of the protein thins the membrane near the transport pathway to facilitate rapid transbilayer lipid movement.
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Structural basis of Ca(2+)-dependent activation and lipid transport by a TMEM16 scramblase.,Falzone ME, Rheinberger J, Lee BC, Peyear T, Sasset L, Raczkowski AM, Eng ET, Di Lorenzo A, Andersen OS, Nimigean CM, Accardi A Elife. 2019 Jan 16;8. pii: 43229. doi: 10.7554/eLife.43229. PMID:30648972<ref>PMID:30648972</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6e1o" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
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[[Category: Aspfu]]
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[[Category: Aspergillus fumigatus Af293]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Accardi, A]]
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[[Category: Accardi A]]
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[[Category: Falzone, M E]]
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[[Category: Falzone ME]]
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[[Category: Ca2+-activated]]
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[[Category: Lipid transport]]
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[[Category: Membrane-reorganization]]
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[[Category: Scramblase]]
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Current revision

afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0

6e1o, resolution 3.59Å

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