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6i60

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Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum

Structural highlights

6i60 is a 2 chain structure with sequence from Dictyoglomus thermophilum H-6-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.743Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B5YC64_DICT6

Publication Abstract from PubMed

alpha-l-Rhamnosidases are catalysts of industrial tremendous interest, but their uses are still somewhat limited by their poor thermal stabilities and selectivities. The thermophilic DtRha from Dictyoglomus thermophilum was cloned, and the recombinant protein was easily purified to homogeneity to afford 4.5 mg/L culture of biocatalyst. Michaelis-Menten parameters demonstrated it to be fully specific for alpha-l-rhamnose. Most significantly, DtRha demonstrated to have a stronger preference for alpha(1 --> 2) linkage rather than alpha(1 --> 6) linkage when removing rhamnosyl moiety from natural flavonoids. This selectivity was fully explained by the difference of binding of the corresponding substrates in the active site of the protein.

Biochemical Characterization of the alpha-l-Rhamnosidase DtRha from Dictyoglomus thermophilum: Application to the Selective Derhamnosylation of Natural Flavonoids.,Guillotin L, Kim H, Traore Y, Moreau P, Lafite P, Coquoin V, Nuccio S, de Vaumas R, Daniellou R ACS Omega. 2019 Jan 24;4(1):1916-1922. doi: 10.1021/acsomega.8b03186. eCollection, 2019 Jan 31. PMID:31459445^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Guillotin L, Kim H, Traore Y, Moreau P, Lafite P, Coquoin V, Nuccio S, de Vaumas R, Daniellou R. Biochemical Characterization of the alpha-l-Rhamnosidase DtRha from Dictyoglomus thermophilum: Application to the Selective Derhamnosylation of Natural Flavonoids. ACS Omega. 2019 Jan 24;4(1):1916-1922. doi: 10.1021/acsomega.8b03186. eCollection, 2019 Jan 31. PMID:31459445 doi:http://dx.doi.org/10.1021/acsomega.8b03186

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6i60"

Categories: Dictyoglomus thermophilum H-6-12 | Large Structures | Daniellou R | Lafite P

@@ Line 3: / Line 3: @@
 <StructureSection load='6i60' size='340' side='right'caption='[[6i60]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6i60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dict6 Dict6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I60 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6i60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyoglomus_thermophilum_H-6-12 Dictyoglomus thermophilum H-6-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I60 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AE3:2-(2-ETHOXYETHOXY)ETHANOL'>AE3</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.743&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DICTH_0289 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=309799 DICT6])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AE3:2-(2-ETHOXYETHOXY)ETHANOL'>AE3</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i60 OCA], [https://pdbe.org/6i60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i60 RCSB], [https://www.ebi.ac.uk/pdbsum/6i60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i60 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/B5YC64_DICT6 B5YC64_DICT6]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Dict6]]
+[[Category: Dictyoglomus thermophilum H-6-12]]
 [[Category: Large Structures]]
-[[Category: Daniellou, R]]
+[[Category: Daniellou R]]
-[[Category: Lafite, P]]
+[[Category: Lafite P]]
-[[Category: Glycoside hydrolase]]
-[[Category: Hydrolase]]
-[[Category: Substrate]]

6i60

From Proteopedia

Current revision

Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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