6yi5
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ==== | + | ==In-situ structure of the trimeric HEF from influenza C by flexible fitting into a cryo-ET map.== |
| - | <StructureSection load='6yi5' size='340' side='right'caption='[[6yi5]]' scene=''> | + | <StructureSection load='6yi5' size='340' side='right'caption='[[6yi5]], [[Resolution|resolution]] 9.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yi5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_C_virus_(C/Johannesburg/1/66) Influenza C virus (C/Johannesburg/1/66)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YI5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yi5 OCA], [https://pdbe.org/6yi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yi5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yi5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.1Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yi5 OCA], [https://pdbe.org/6yi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yi5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yi5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The lipid-enveloped influenza C virus contains a single surface glycoprotein, the haemagglutinin-esterase-fusion (HEF) protein, that mediates receptor binding, receptor destruction, and membrane fusion at the low pH of the endosome. Here we apply electron cryotomography and subtomogram averaging to describe the structural basis for hexagonal lattice formation by HEF on the viral surface. The conformation of the glycoprotein in situ is distinct from the structure of the isolated trimeric ectodomain, showing that a splaying of the membrane distal domains is required to mediate contacts that form the lattice. The splaying of these domains is also coupled to changes in the structure of the stem region which is involved in membrane fusion, thereby linking HEF's membrane fusion conformation with its assembly on the virus surface. The glycoprotein lattice can form independent of other virion components but we show a major role for the matrix layer in particle formation. | ||
| + | |||
| + | In situ structure and organization of the influenza C virus surface glycoprotein.,Halldorsson S, Sader K, Turner J, Calder LJ, Rosenthal PB Nat Commun. 2021 Mar 16;12(1):1694. doi: 10.1038/s41467-021-21818-9. PMID:33727554<ref>PMID:33727554</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yi5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Halldorsson S]] |
| + | [[Category: Rosenthal PB]] | ||
Current revision
In-situ structure of the trimeric HEF from influenza C by flexible fitting into a cryo-ET map.
| |||||||||||
