7dqv
From Proteopedia
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==Crystal structure of a CmABCB1 mutant== | ==Crystal structure of a CmABCB1 mutant== | ||
| - | <StructureSection load='7dqv' size='340' side='right'caption='[[7dqv]]' scene=''> | + | <StructureSection load='7dqv' size='340' side='right'caption='[[7dqv]], [[Resolution|resolution]] 2.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DQV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7dqv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyam1 Cyam1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DQV FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqv OCA], [https://pdbe.org/7dqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqv RCSB], [https://www.ebi.ac.uk/pdbsum/7dqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqv ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CYME_CMD148C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=280699 CYAM1])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqv OCA], [https://pdbe.org/7dqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqv RCSB], [https://www.ebi.ac.uk/pdbsum/7dqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CmABCB1 is a homologue of human P-glycoprotein, which extrudes various substrates by iterative cycles of conformational changes between the inward- and outward-facing states. Comparison of the inward- and outward-facing structures of CmABCB1 suggested that pivotal joints in the transmembrane domain regulate the tilt of transmembrane helices. Transmembrane helix 1 (TM1) forms a tight helix-helix contact with TM3 at the TM1-3 joint. Mutation of Gly132 to valine at the TM1-3 joint, G132V, caused a 10-fold increase in ATPase activity, but the mechanism underlying this change remains unclear. Here, we report a crystal structure of the outward-facing state of the CmABCB1 G132V mutant at a 2.15 A resolution. We observed structural displacements between the outward-facing states of G132V and the previous one at the region around the TM1-3 joint, and a significant expansion at the extracellular gate. We hypothesize that steric hindrance caused by the Val substitution shifted the conformational equilibrium toward the outward-facing state, favoring the dimeric state of the nucleotide-binding domains and thereby increasing the ATPase activity of the G132V mutant. | ||
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| + | The crystal structure of the CmABCB1 G132V mutant, which favors the outward-facing state, reveals the mechanism of the pivotal joint between TM1 and TM3.,Matsuoka K, Nakatsu T, Kato H Protein Sci. 2021 Mar 8. doi: 10.1002/pro.4058. PMID:33683740<ref>PMID:33683740</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7dqv" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Cyam1]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kato H]] | + | [[Category: Kato, H]] |
| - | [[Category: Matsuoka K]] | + | [[Category: Matsuoka, K]] |
| - | [[Category: Nakatsu T]] | + | [[Category: Nakatsu, T]] |
| + | [[Category: Multidrug resistance abc transporter membrane protein]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 08:33, 21 April 2021
Crystal structure of a CmABCB1 mutant
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